蚕豆根尖微核技术测定美洲大蠊和九香虫混合成分的抗突变性  

Micronucleus Test of Vicia Faba L. Root Tip Cells to Assess the Anti-mutation Property of Periplaneta Americana and Aspongopus Chinensis Dallas Mixture

作　　者：宋文涛[1] 马志敏[1] 季守莲[1] 刘光明[2] 施贵荣[2] 

机构地区：[1]大理学院公共卫生学院 [2]大理学院药学院,云南大理671000

出　　处：《癌变．畸变．突变》2008年第6期475-476,489,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：大理学院科研基金资助项目(2007X15);国家自然科学基金(30560181)

摘　　要：背景与目的:了解美洲大蠊和九香虫混合成分的抑突变作用。材料与方法:将来自大理市周边七里桥、小关邑的两种蚕豆各分为环磷酰胺组、阴性对照组、美洲大蠊和九香虫混合成分组、美洲大蠊和九香虫混合成分+环磷酰胺组,共4组,利用蚕豆根尖微核技术,测定美洲大蠊和九香虫混合成分对环磷酰胺所诱导突变的抑制作用。结果:美洲大蠊和九香虫混合成分+环磷酰胺处理组的根尖细胞微核率明显低于环磷酰胺组(P<0.01),其微核抑制率分别为60.5(七里桥蚕豆)和52.5(小关邑蚕豆)。结论:美洲大蠊和九香虫混合成分可以降低环磷酰胺诱导的蚕豆根尖细胞的微核率,具有抑突变作用。BACKGROUND AND AIM： To investigate the anti-mutation property of the Periplaneta Americana and Aspengopus Chinensis Dallas mixture. MATERIALS AND METHODS： Broad beans from Qiliqiao village and Xiaoguanyi village were divided into 4 groups, cyclophosphamide group, negative control group, Periplaneta Americana and Aspongopus Chinensis Dallas mixture group, Periplaneta Americana and Aspongopus Chinensis Dallas mixture ＋ cyclophosphamide group.The purpose was to assess the anti-mutation property of Periplaneta Americana and Aspongopus Chinensis Dallas mixture by means of micronucleus test of Vicia Faba L.root tip cells. RESULTS： Compared with the cyclophosphamide group, the rates of micronucleus in the Periplaneta Americana and Aspongopus Chinensis Dallas mixture ＋ cyclophosphamide group were lower. The inhibitory rates of Periplaneta Americana and Aspongopus Chinensis Dallas mixture ＋ cyclophosphamide group were 60.5% （Qiliqiao） and 52.5% （Xiaoguanyi） . CONCLUSION： Periplaneta Americana and Aspongopus Chinensis Dallas mixture could effectively inhibit the mutation induced by cyclophosphamide.

关 键 词：美洲大蠊 九香虫 蚕豆 微核 环磷酰胺 抗突变 

分 类 号：R730.1[医药卫生—肿瘤]