mTOR抑制剂对小鼠受精卵卵裂以及MPF活性的影响  

The effect of mTOR inhibitor on the cleavage and MPF activity in mouse fertilized eggs first mitosis

作　　者：孙炳奇[1] 邱攀[1] 钟红红 于秉治[3] 

机构地区：[1]沈阳市第五人民医院,110023 [2]山东省平都市呼吸病防治所 [3]中国医科大学基础医学院生物化学与分子生物学教研室

出　　处：《辽宁医学杂志》2008年第6期285-288,共4页Medical Journal of Liaoning

摘　　要：目的观察哺乳动物雷帕霉素靶(mTOR)抑制剂对小鼠受精卵第一次卵裂及第一次有丝分裂中MPF活性的影响。方法在小鼠受精卵中加入雷帕霉素后,显微镜观察卵细胞分裂情况,计算卵裂率;用液闪计数仪测定各组分别加入雷帕霉素和渥曼青霉素后的MPF活性;用免疫电泳方法检测加入雷帕霉素后cyclin B的表达。结果加入mTOR抑制剂后,卵裂率下降,MPF活性在小鼠受精卵第一次分裂中未见升高,卵细胞中cyclin B的表达下降。结论mTOR抑制剂可抑制小鼠受精卵第一次卵裂,同时抑制小鼠受精卵第一次分裂时MPF活性的升高。Objective To research the effects of roTOR inhibitor on the cleavage and MPF activity in mouse fertilized eggs first mitosis. Methods Observe the situation of cleavage under microscope after add rapamycin in mouse fertilized eggs and count the cleavage rate; count MPF activity with Beckman liquid scintillation counter after add rapamycin and wortmannin in each group respectively; detect the expression of cyclin B with western blot after add rapamycin. Results The cleavage rate decrease, MPF activity does not increase in first mitosis and the expression of cyclin B decrease after roTOR inhibitor is added in mouse fertilized eggs. Conclusion The inhibitor of roTOR can inhibit the cleavage of mouse fertilized eggs and it can inhibit MPF activity in zygotes first motosis.

关 键 词：受精卵 MTOR MPF 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R96[医药卫生—基础医学]