PCR-Dotblot杂交法直接检测临床病原菌的报告  

Report for direct detection and identification of gram positive and negative bactetia from clinical specimens

作　　者：徐芸[1] 杨瑞馥[1] 郭兆彪[1] 李继昌[1] 

机构地区：[1]河南医科大学第一附属医院 [2]军事医学科学院微生物流行病学研究所

出　　处：《中华医院感染学杂志》1998年第1期14-16,共3页Chinese Journal of Nosocomiology

摘　　要：目的为了寻找临床病原菌系统，检测和鉴别的有力手段。方法用真细菌保守的１６ＳｒＲＮＡ基因为模板，以ＰＣＲ－Ｄｏｔｂｌｏｔ杂交的方法检测临床病原菌。结果它将１６ＳｒＲＮＡ基因的广谱性和变异性并存之特点和ＰＣＲ－Ｄｏｔｂｌｏｔ杂交的敏感性结合起来，对该法的建立进行了探讨，并初步用于临床感染的检测。结论为细菌通用检测法的建立提供了基础。Objective To fine the method of defecting clinical pathogens. Method A polymerease chain reaction targeted conserved sequence of eubacterial 16S rRNA geme, combined with dot blot hybridization was used to detect clinical pathogens. Results this method relyed on ubiquity and diversity of 16S rRNA and sensitivity of dot-blot hybridization. Conclusion applied the basis for developing the general diagnostic system for clinical pathogens. ln this paper such a method was developed and applied to diagnose of clinlcal lnfections.

关 键 词：16SRRNA基因 PCR DOT 病原菌 杂交法 检测 

分 类 号：R372[医药卫生—病原生物学]