原发性肝细胞癌患者外周血AFP mRNA的表达及其临床意义  

Detection of Alpha-fetoprotein mRNA in Peripheral Blood of Patients with Primary Hepatocellular Carcinoma by RT-PCR

作　　者：张华[1] 陈华[1] 李永兴[1] 项明洁[1] 

机构地区：[1]上海瑞金医院卢湾分院放免检验科,上海200020

出　　处：《标记免疫分析与临床》2008年第6期340-342,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：采用逆转录聚合酶链式反应(RT-PCR)检测78例原发性肝细胞癌(HCC)患者及109例其它疾病组患者外周血AFP mRNA的表达,同时用微粒子酶免疫法检测血清AFP水平。HCC外周血AFP mRNA的阳性率为61.5%,其它疾病组阳性率为1.8%,对照组均为阴性。HCC患者外周血中,AFP mRNA的阳性率与肝内静脉癌栓形成、肝内外转移以及与肿瘤分期、大小有关。作为肿瘤微转移检测的灵敏指标,外周血AFP mRNA能更好反映肿瘤的早期转移。To establish a method for detecting tumor micrometastasis in peripheral blood. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect Alpha-fetoprotein mRNA （AFP mRNA） in peripheral blood of 78 patients with primary hepatocellular carcinoma （HCC）. The serum AFP level was determined by microparticle enzyme immunoassay. The results showed that the AFP mRNA positive rate in patients with HCC, patients with other diseases, and the normal healthy people were 61.54% , 1.83% and 0 respectively. The presence of AFP mRNA in blood of HCC patients was correlated with portal vein invasion, intrahepatic and extrahepatic metastasis, tumor size and TNM staging. RT-PCR is a sensitive method for detecting AFP mRNA in peripheral blood of HCC patients, which might predict early metastasis of cancer cells.

关 键 词：逆转录聚合酶链式反应 原发性肝细胞癌 甲胎蛋白MRNA 

分 类 号：R735.7[医药卫生—肿瘤]