P2X7 Receptor Mediated Growth-Inhibitory Effect in KG1a Cell Line  

作　　者：Xiujun Zhang Lijun Meng 

机构地区：[1]Department of Biological Sciences, North China Coal Medical College, Tangshan 063000, Heibei Province, China [2]Department of Environment ＆ Chemical Engineering, Tangshan College, Tangshan 063000. Heibei Province, China

出　　处：《Chinese Journal of Clinical Oncology》2008年第6期400-406,共7页中国肿瘤临床（英文版）

基　　金：This work was supported by a grant from the National NaturaI Science Foundation of China(No.30671092)

摘　　要：OBJECTIVE This study was conducted to investigate ATP- induced growth inhibition in human leukemic cells KG1a. METHODS ATP inhibited cell growth was analyzed by MTS assay. Externalization of phosphatidylserine could be detected by Annexin-V-FITC apoptosis staining after activation of the P2X7 receptor. P2X7 mediated pore formation was detected in KGla cells by Yo-Pro-1 uptake assay. RESULTS ATP inhibited cell growth in a dose-dependent manner. The cytotoxic effect could be blocked by P2X7 antagonists, oxidized ATP （oATP） and KN62. Externalization of phosphatidylserine could be detected in a time-dependent manner. P2X7 mediated pore formation could be detected in KG1a cells. These effects could not be observed in P2X7 null Ramos cells. CONCLUSION The results and our previously reports that mRNA, protein expression and calcium response of the P2X7 receptor in KGla cells, suggested that extracellular ATP effectively induces growth inhibition through apoptosis in KGla cells by activation of P2X7 receptor, and that may be mediated by extracellular Ca^2＋ in ux and pore formation.This study was conducted to investigate ATP-induced growth inhibition in human leukemic cells KGla.METHODS ATP inhibited cell growth was analyzed by MTSassay.Externalization of phosphatidylserine could be detected byAnnexin-V-FITC apoptosis staining after activation of the P2X7 re-ceptor.P2X7 mediated pore formation was detected in KGla cellsby Yo-Pro-1 uptake assay.RESULTS ATP inhibited cell growth in a dose-dependent man-ner.The cytotoxic effect could be blocked by P2X7 antagonists,oxidized ATP (oATP) and KN62.Externalization of phosphatidyl-serine could be detected in a time-dependent manner.P2X7 medi-ated pore formation could be detected in KGla cells.These effectscould not be observed in P2X7 null Ramos cells.CONCLUSIONThe results and our previously reports thatmRNA,protein expression and calcium response of the P2X7receptor in KGla cells,suggested that extracellular ATP effectivelyinduces growth inhibition through apoptosis in KGla cells byactivation of P2X7 receptor,and that may be mediated by extracel-lular Ca^(2+)in@ux and pore formation.

关 键 词：P2X7 receptor growth inhibition APOPTOSIS extracellular ATP leukemia cells. 

分 类 号：R733.7[医药卫生—肿瘤]