NH_4^+对混合菌种的生长及光合产氢的影响  

THE EFFECTS OF NH_4^+ CONCENTRATION ON GROWTH AND PHOTOTROPHIC HYDROGEN PRODUCTION OF MIXED CULTURES

作　　者：张立宏[1] 周俊虎[1] 陈明[1] 程军[1] 刘建忠[1] 岑可法[1] 

机构地区：[1]能源清洁利用国家重点实验室(浙江大学),杭州310027

出　　处：《太阳能学报》2008年第12期1546-1552,共7页Acta Energiae Solaris Sinica

基　　金：国家自然科学基金(50406022);国家高技术研究发展计划(863)项目(2006AA05Z122);全国优秀博士学位论文作者专项资金资助项目(200437);霍英东高等院校青年教师优选资助项目(104021);浙江省科技攻关计划项目(2006C33049)

摘　　要：针对 NH_4^+浓度对混合菌种的生长与光合产氢的影响进行了试验研究。通过对3种生长模型的对比,确定了修正的 Gompertz 方程作为描述混合菌种光合生长的模型。结果表明 NH_4^+对混合菌种的最大菌浊度影响不大。通过 pH 值的控制与不控制时不同 NH_4^+浓度的对比产氢试验,发现不控制 pH 值时,NH_4^+对?昆合菌种光合产氢的抑制作用显著;而控制 pH=7.0时,2.8mmol/L的 NH_4^+浓度比无 NH_4^+时的产氢率高、产氢速率快,而后随着NH_4^+浓度的继续增大,产氢率不断减少。这表明控制 pH 时,适当低浓度的 NH_4^+可以提高混合菌种的光合产氢率以及产氢速率。无论是否控制 pH 值,只有 NH_4^+被菌体的生长所消耗掉,才开始产氢,这表明 NH_4^+对产氢的抑制作用在一定范围内是可恢复的。同时通过混合菌种与纯菌株 Rh.Palustris Z02的对比试验发现,无论对于生长还是产氢,NH_4^+对混合菌种的影响均小于对纯菌株 Rh.Palustris Z02的影响。Batch experiments were conducted to investigate the effects of NH4^＋concentration on growth and phototrophic hydrogen production of mixed cultures. Compared with Logistic and Gompertz equation, the modified Gompertz equation was the best model for describing the growth of mixed cultures. The results show that the cell biomass of mixed cultures is not readily affected by NH4^＋ . Contrast test of controlled and uncontrolled pH was carried out. The results show that the inhibitory effect of NH4^＋ is obvious when pH is uncontrolled. But hydrogen yield and hydrogen production rate （HPR） of NH4^＋ concentration of 2.8mmol/L are higher than those of no NH4^＋ when pH is controlled. This indicats that appropriate low NH4^＋ concentration could enhance hydrogen yield and HPR. For growth and hydrogen production, the effect of NH4^＋ concentration on mixed cultures is lesser than pure culture Rh. Palustris Z02.

关 键 词：光合产氢 生长 混合菌种 NH4^+浓度 

分 类 号：TK511.5[动力工程及工程热物理—热能工程]