Bacillus subtilis ZJUTZY产γ-聚谷氨酸的分子量分布及其絮凝特性研究  

A range of molecular weight and flocculating characters of γ-poly glutamic acid produced by Bacillus subtilis ZJUTZY

作　　者：张毅[1] 汪新[1] 邱乐泉[1] 朱雪娜[1] 钟卫鸿[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2008年第6期647-650,共4页Journal of Zhejiang University of Technology

基　　金：浙江省生物化工重中之重学科开放基金资助项目(200801)

摘　　要：利用自行筛选获得的一株产γ-聚谷氨酸枯草芽孢杆菌Bacillus subtilis ZJUTZY，制备了γ-聚谷氨酸（γ-PGA）样品．考察了其分子量分布和对Ca（OH）2的絮凝活性，结果显示，该菌所产γ-PGA的分子量分布范围为40～110kDa，其最佳絮凝条件为：γ-PGA添加浓度为0．08g／L，絮凝体系的pH为6．0，适宜温度为40℃．添加Mg^2＋，Ca^2＋，K^＋能促进其絮凝活性，而添加Fe^3＋，Co^2＋，Mn^2＋则对絮凝活性有抑制作用．Bacillus subtilis ZJUTZY产γ-PGA的最高絮凝率可达46．99％．In this study, molecular weight and flocculating activity of a bioflocculants poly-γ- glutamic acid（γ-PGA）obtained by a new isolate named Bacillus subtilis ZJUTZY were studied. The result showed that molecular weight of γ-PGA was in the range from 40 kDa to 110 kDa. The optimum flocculating conditions were as follows： γ-PGA additional concentration 0.08 g/L, system pH 6. 0, temperature 40 ℃. It was found that Mg^2＋, Ca2^＋, K^＋ could improve the flocculating activity of γ-PGA. However, Fe^3＋, Co^2＋, Mn^2＋ would inhibit the flocculating activity of γ-PGA. The maximal flocculating efficiency could, reach 46.99%.

关 键 词：聚Γ-谷氨酸 生物絮凝剂 枯草芽孢杆菌 

分 类 号：Q939.97[生物学—微生物学]