玉米小斑病菌复壮培养基的筛选  

Screening of Rejuvenation Medium with Helminthosporium maydis Race C(HMC)

作　　者：李秀丽[1,2] 董文琦[3] 李运朝[1] 贾银锁[1] 马春红[1] 王立安[2] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北石家庄050051 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北省农林科学院,河北石家庄050051

出　　处：《河北农业科学》2008年第12期38-39,共2页Journal of Hebei Agricultural Sciences

基　　金：河北省自然科学基金资助项目(C2006000744);国际科技合作资助项目(2006DFB02480);河北省科学技术研究与发展计划资助项目(07297162D)

摘　　要：选用7种培养基对出现退化的玉米小斑病菌进行复壮培养。结果显示:通过V8培养基和玉米苗浸汁培养基获得的复壮菌株,菌落形态表现为菌丝黑密粗壮,且生长速度(分别为6.46 mm/d和6.33 mm/d)、产孢量(分别为2.72×106个/mL和1.92×106个/mL)和致病力等均得到恢复与提高。复壮后的病毒粗提液,对C和N细胞质产生较强的专效性毒害,反映出对菌株复壮的高度有效性。Seven rejuvenation mediums of HMC were used in this paper, and two superior mediums V8-Juice agar medium and corn seedlings-cooked agar medium were obtaind. The strain grew strongly in these two mediums. The mycelium was dense and thick, and the growth rate were 6. 46 mm/d and 6. 33 mm/d respectively. Sporulation quality were 2. 72 × 10^6 A/mL and 1.92 × 10^6 A/mL respectively. The rejuvenated viral extraction had a specific effect of poisoning on C and N cytoplasm, whith reflected the strain on the rejuvenation had the high degree of effectiveness.

关 键 词：玉米小斑病菌 复壮培养基 产孢量 致病力 

分 类 号：S432.1[农业科学—植物病理学]