酶法处理对油菜花粉萌发孔通透性的改善  

Effect of Enzymatic Treatments on Permeability of Germ Pores of Rape Pollens

作　　者：董捷[1,2] 李春阳[3] 张红城[1,2] 李慧岩[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]农业部国家蜂产品加工专业分中心,北京100093 [3]江苏省农科院农产品加工研究所,江苏南京210014

出　　处：《食品与生物技术学报》2008年第6期103-106,共4页Journal of Food Science and Biotechnology

基　　金：国家“十一五”科技支撑计划重点项目(2006BAD14B07-5-0);农业部基本科研业务费专项课题(200703);中国农业科学院蜜蜂研究所所长基金项目(200605)

摘　　要：研究了经酶法处理的油菜花粉的营养物质的溶出情况和萌发孔打开情况。结果表明:用中温淀粉酶和复合纤维素酶处理花粉后,每克花粉上清液中可溶性糖含量最高可达到(0.365±0.017)g,与空白相比提高了53%;用复合蛋白酶和复合纤维素酶处理花粉后,蛋白质溶解指数达到最高为0.66±0.05;通过显微镜可以观察到经过复合蛋白酶和复合纤维素酶处理后,花粉萌发孔明显变大。This manuscript studied the effect of enzymatic treatments on permeability of germ pores of rape pollens. The results indicated that the content of soluble sugar of pollens treated byα-amylase of BAN and cellulase of celluclast from Novozymes was （0.365±0.017） g, increasing by 53 % compared with that of the control. The solution index of pollens treated by protease of protamex and cellulose of celluclast from Novozymes was chieved at 0. 66±0. 05. And germ pores of pollens treated by protease of protamex and cellulose of celluclast opened the greatest.

关 键 词：油菜花粉 酶法 萌发孔 

分 类 号：S896.4[农业科学—特种经济动物饲养]