具有质膜ATP酶活力的大豆细胞壁碎片的制备  

Properties of ATPase activity associated with cell wall fractopm from hypocotyls of soybean

作　　者：陈秉初[1] 王明鑫[1] 方芳[1] 朱诚[1] 

机构地区：[1]浙江师范大学生物系,浙江农业大学基础课部

出　　处：《浙江农业大学学报》1998年第1期82-84,共3页

摘　　要：通过反复洗涤与低速离心（１０００×ｇ，１０ｍｉｎ），从大豆下胚轴得到的细胞壁碎片具有ＡＴＰ酶活力．酶活依赖于Ｍｇ２＋的存在，受Ｋ＋刺激和钒酸钠的强烈抑制．经加ＫＩ的悬浮液洗涤，碎片ＡＴＰ酶受叠氮化钠抑制很少，几乎不受硝酸盐抑制．表明所得的ＡＴＰ酶基本上为质膜ＡＴＰ酶．本方法简化了质膜的制备过程，在质膜及质膜ＡＴＰ酶的生理研究中有较高的应用价值．Cell wall fractions were prepared from Glycine max hypocotyls by low speed centrifugation (1　000×g, 10　min) of homogenate and repeated washing. The fractions contained ATPase activity that is dependent on Mg2+, stimulated by KCl and strongly inhibited by vanadate. Some inhibition of the activity was observed by azide but this inhibition was removed after washing with KI. And little inhibition was observed by KNO3. The results suggest that the ATPase activity associated with the cell wall fractions is predominantly of plasma membrane origin. Thus this simple method provides a quick and easy means of studying plasma membrane ATPase activity.

关 键 词：质膜 质膜ATP酶 大豆 

分 类 号：S565.101[农业科学—作物学]