甲基对硫磷降解菌L1的分离、鉴定及降解酶基因的克隆  

Isolation,Characterization of Methyl-parathion Degrading Strain L1 and Cloning of the mpd Gene

作　　者：李莹莹[1] 李文[1] 张琛[1] 闫艳春[2] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]中国农业科学院研究生院,北京100081

出　　处：《生物技术通报》2008年第6期128-131,共4页Biotechnology Bulletin

基　　金：国家高技术研究发展计划(863计划)(2008AA10Z402、2006AA10Z402)

摘　　要：从农药厂的污水处理池中分离到一株能高效降解甲基对硫磷的菌株L1,L1能以甲基对硫磷为惟一碳源、氮源和能源生长;经生理生化实验和16s rRNA同源性分析,初步将L1归为节杆菌属(Arthrobacter sp.)。紫外分光光度法对L1的降解性能研究表明,L1能在12h内将50mg/L的甲基对硫磷完全降解;用PCR方法克隆其甲基对硫磷降解酶基因mpd,这是迄今首次从革兰氏阳性菌中克隆到的mpd基因。Strain L1,capable of utilizing methyl-parathion as sole carbon,nitrogen and energy source,was isolated from the activated sludge by enrichment technique.As the result,the strain L1 was identified as Arthrobacter sp.by biochemical tests and analysis of the partial 16S rDNA sequence.Degradation tests of MP with spectrophotometry revealed that L1 could degrade 50mg/L MP completely in 12h.The methyl-parathion degrading mpd gene was cloned.This is the first report of isolating the mpd gene from the positive strain.

关 键 词：甲基对硫磷 节杆菌属 甲基对硫磷降解酶基因 

分 类 号：X172[环境科学与工程—环境科学] TQ453.22[化学工程—农药化工]