乙型肝炎病毒基因生物信息学分析及特异片段的制备  

Bioinformatic Analysis of HBV Gene and Preparation of Specific Fragment

作　　者：徐晓雪[1] 房凯[1] 钟连声[1] 潘忠诚[1] 李超[1] 胥威[1] 赵雨杰[1] 

机构地区：[1]中国医科大学基础医学院生物芯片中心,沈阳110001

出　　处：《中国医科大学学报》2008年第5期583-585,共3页Journal of China Medical University

基　　金：辽宁省教育厅高校科研基金资助项目(202011001)

摘　　要：目的制备HBV基因分型芯片模板。方法通过不同基因型HBV全基因组的序列比对,选取6个碱基位点作为分型位点,应用重叠区扩增基因拼接法(SOEing法)对C型HBV片段进行点突变。结果在所选位点上成功引入点突变。结论SOEing法是一种简便引入点突变的方法,可以应用此法制备HBV基因分型检测基因所需的模板DNA。Objective To prepare HBV DNA fragments for HBV genotypes used as templates. Methods Six bases were chosen for genotyping by comparing different HBV genome sequences. The fragments of HBV genotype C were mutated by using gene splicing by overlap extension （gene SOEing）. Results The chosen sites were successfully mutated. Conclusion Gene SOEing is a convenient way to introduce mutation, and this method can be used to prepare HBV specific sequences that can be used as templates.

关 键 词：重叠区扩增基因拼接法 乙型肝炎病毒 基因分型 

分 类 号：R512.6[医药卫生—内科学]