伏马菌素B_1单抗制备及其ELISA方法的研究  

Preparation of Monoclonal Antibodies against Fumonisin B_1 in Maize

作　　者：宫慧芝[1] 计融[2] 杨军[1] 江涛[2] 

机构地区：[1]中国医科大学公共卫生学院卫生检验学教研室,沈阳110001 [2]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《中国医科大学学报》2008年第5期629-630,共2页Journal of China Medical University

基　　金：国家"十五"科技重大专项基金资助项目(2001BA804A3205)

摘　　要：目的建立应用单克隆抗体的伏马菌素B(1FB1)的间接竞争酶联免疫吸附检测方法。方法利用B淋巴细胞杂交瘤技术制备抗FB1单克隆抗体,建立FB1间接竞争酶联免疫吸附方法。结果制备了稳定分泌抗FB1毒素单克隆抗体的杂交瘤细胞株,分泌的抗体为IgG1亚类,亲和常数为6.72×109L/mol。该方法最低检出浓度为5μg/L,校正曲线的线性范围50～500μg/L,线性方程Y=-0.582X+1.793(r=0.99,P<0.05)。回收率在71.89%～112.95%之间,平均回收率为100.23%。实验室内的变异系数和实验室间的变异系数均小于20%。结论分泌抗FB1单克隆抗体的杂交瘤细胞株稳定,检测方法快速、灵敏、特异。Objective To prepare monoclonal antibodies against fumonisin B1 （FB1） and develop the enzyme-linked immtmosorbant assay （ELISA） for detecting FB1. Methods The monoelonal antibodies against FB1 were prepared by using B cell hybridoma technique,and ELISA was established for detecting FB1. Results Monoclonal antibody identified in ELISA was IgG1 subtype, and the affinity constant was 6.72×10^9 L/tool. The limit of detectable concentration of FB1 was 5μg/L. The linear range was from 50 to 500 μg/L （linear equation Y= - 0.582X＋ 1.793,r =0.99,P〈 0.05）. The recoveries of spiked maize ranged between 71.89% and 112.95% ,and the mean recovery was 100.23%. Conclusion The hybridoma cell lines excreting monoelonal antibodies against FB1 are established in this study, and the detection method is rapid, sensitive, and specific.

关 键 词：伏马菌素B1 单克隆抗体 酶联免疫吸附试验 

分 类 号：R155.5[医药卫生—营养与食品卫生学]