七叶皂苷钠对大鼠局灶性脑缺血再灌注后MMP-9和LN表达的影响  

Effect of sodium β-aescin on MMP-9 and LN expression in rats after focal cerebral ischemia and reperfusion

作　　者：张韶华[1] 韩文江[1] 李晓蕾[1] 王秀丽[1] 

机构地区：[1]哈尔滨医科大学大庆校区病理教研室,黑龙江大庆163319

出　　处：《西安交通大学学报（医学版）》2008年第6期621-624,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的研究七叶皂苷钠(sodium β-aescin)对大鼠局灶性脑缺血再灌注后基质金属蛋白酶-9(matrix metallopro-teinase-9,MMP-9)和层黏连蛋白(laminin,LN)表达影响,为深入探讨七叶皂苷钠对脑的保护机制提供实验依据。方法制备大鼠局灶性脑缺血再灌注模型,随机分为正常组、假手术组、缺血再灌注组和缺血再灌注七叶皂苷钠治疗组,后两组又分为缺血2h后再灌注3、6、12、24、72h和7d,共6个时间点,每时间点4只;用免疫组织化学法和图像分析观察其缺血区LN和MMP-9的表达变化。结果缺血再灌组MMP-9在神经元的表达于再灌注6h开始增加,12h达高峰,24h又降低,7d最低,治疗组MMP-9于再灌注同一时相有显著减少;LN在微血管的表达于再灌注12h明显减少,24h时达低峰,72h开始回升,治疗组在同一时相LN表达显著增多。结论MMP-9与LN可能参与脑血管源性脑水肿的形成发展,缺血再灌注早期七叶皂苷钠治疗通过减少MMP-9的表达,增加LN的表达对缺血再灌注脑起保护作用。Objective To study the effect of sodium β-aescinon the matrix metalloproteinase-9 （MMP-9） and laminin （LN） expression in rats after focal cerebral ischemia and reperfusion, and provide experimental evidence for the relationship of MMP-9 and LN expression change with the neuroprotection of sodium β-aescin. Methods The Spraque-Dawley （SD） rat model of focal cerebral ischemia and reperfusion of cerebral middle artery was prepared. Fifty-six rats were randomly divided into the normal, sham, ischemia and reperfusion, as well as ischemia and reperfusion-treated with sodium β-aescin groups. The latter two were further divided into six subgroups according to reperfusion time interval of 3 h, 6 h, 12 h, 24 h, 72 h and 7 d after brain ischemia, with 4 rats in each subgroup. Immunohistochemical staining and image analysis were used to measure the expression of MMP-9 and LN in the ischemic region. Results The expression of MMP-9 started to increase at 6 h, reached the peak at 12 h, decreased in 24 h, and reached the lowest on 7 d in cerebral ischemia and reperfusion group. The expression of MMP-9 in the same time groups of the cerebral ischemia and reperfusion-treated with sodium β-aescin group all obviously decreased （P%0.05）. The expression of LN in the microvessels started to decrease at 3 h, reached the lowest at 24 h, and increased at 72 h in the cerebral ischemia and reperfusion group. The expression of LN in the same time groups of the cerebral ischemia and reperfusion-treated with sodium β-aescin group all elevated （P〈0.05）.Conclusion MMP-9 and LN probably have a close relationship with the formation and progression of vascular brain edema. Sodium β-aescin can help recovery of damaged brain by decreasing MMP-9 expression and increasing LN expression in rats after early brain ischemia and reperfusion.

关 键 词：七叶皂苷钠 基质金属蛋白酶-9 层黏连蛋白 脑缺血再灌注 大鼠 

分 类 号：R734[医药卫生—肿瘤]