不同加载条件下的微螺钉支抗种植体骨界面的组织学观察  

Histological observation of microscrews anchorage implant-bone interface loaded with different orthodontic condition

作　　者：姜晓红[1] 张扬[2] 韩雪[3] 刘继辉[1] 马天驰[1] 

机构地区：[1]沈阳市口腔医院正畸科,辽宁沈阳110002 [2]中国医科大学口腔医学院正畸科,辽宁沈阳110002 [3]沈阳军区总医院口腔科,辽宁沈阳110016

出　　处：《上海口腔医学》2008年第6期621-624,共4页Shanghai Journal of Stomatology

基　　金：辽宁省自然科学基金(20042076);辽宁省教育厅高校科研项目(A05L459)~~

摘　　要：目的:研究微螺钉支抗种植体在不同加载力值、加载时机与颌骨的结合程度及种植体的稳定性。方法:在3只杂种犬的上、下颌骨分别植入36枚钛合金微螺钉种植体,根据植入颌骨的部位(左、右侧)分为即刻加力组和延期加力组。植入犬左侧颌骨内的延期加力组种植体暂时不加力,4周后与植入犬右侧颌骨内的即刻加力组同时加力。每侧近中种植体不加力,远中2枚种植体相互加力,上颌骨加载1.96N力,下颌骨加载3.92N力。第13周处死动物。标本常规脱钙,行BMP-2免疫组化染色,利用图像分析方法测定骨界面区BMP-2的平均灰度值,分析种植体与骨组织的结合情况。采用SPSS10.0软件包对数据进行t检验和方差分析。结果:即刻加力组(1.96N力、3.92N力)、延期加力组(1.96N力、3.92N力)与非加力组的种植体骨界面BMP-2平均灰度值均有显著性差异(P<0.05),即刻加力组与延期加力组之间平均灰度值无显著性差异(P>0.05)。结论:即刻加载、延期加载正畸矫治力均有助于提高微螺钉支抗种植体与周围骨组织骨性结合的能力。正畸力的加载时机对微螺钉种植体稳定性的影响较小。PURPOSE： To study the stability of the microscrew implant anchorage under different loading force and different timing and observe the integration of the implant to the bone. METHODS： Thirty-six titanium alloy microscrew implants were implanted into three dogs＇ maxilla and mandible. The implants were divided into two groups： one with immediate implant loading and the other with delayed implant loading. In the delayed loading group, the microscrew implants that were implanted into the right maxilla and mandible were unloaded temporarily. Four weeks later, these dogs, together with the immediate loading group, whose microscrew implants were implanted into the left maxilla and mandible, were loaded at the same time. The mesial screw was unloaded. Two distal screws were loaded with force： 1.96N on the maxilla, and 3.92N on the mandibular. After 12 weeks, the dogs were sacrificed. The specimen were fixed and decalcified. Subsequently, immunohistochemical stain for BMP-2 was employed. The specimen were assessed with image analysis system to analyze the average grey scale. The data were analyzed using Student＇s t test and one-way ANOVA with SPSS 10.0 software package. RESULTS： BMP-2 staining showed that there was significant difference between the immediate loading group（1.96N,3.92N）, delayed loading group（1.96N,3.92N） and non-loading group at average gray value （P〈0.05）. But there was no significant difference between the immediate loading group and delayed loading group （P〉0.05）. CONCLUSIONS： Both the immediate and the delayed implant loading can strengthen the attachment and integration of the implant to the bone. The timing of the loading force has no significant impact on the stabilization of the microserew implants.

关 键 词：骨形成蛋白 微螺钉支抗种植体 即刻加载 延期加载 稳定性 

分 类 号：R783.6[医药卫生—口腔医学]