染料木黄酮后处理对人胃黏膜上皮细胞缺氧/复氧损伤的保护作用  

Protective effect of genistein postconditioning on hypoxia/reoxygenation injury in human gastric epithelial cells

作　　者：李豫[1] 张建福[1] 张咏梅[1] 刘美静[1] 石玥[1] 马小波[1] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221002

出　　处：《第四军医大学学报》2008年第24期2229-2231,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30570671)

摘　　要：目的:研究染料木黄酮后处理对人胃黏膜上皮细胞缺氧/复氧损伤的影响.方法:采用人胃黏膜上皮细胞系建立缺氧/复氧损伤模型.实验分为常氧对照组、缺氧/复氧组、染料木黄酮后处理组、DMSO溶剂对照组.各组分别于复氧2,4,8,16h结束后用MTT法检测细胞存活率;并采用缺氧2h/复氧4h模型做Hoechst33258染色观察细胞凋亡情况.结果:MTT检测显示,缺氧/复氧组细胞存活率分别为(64.1±1.7)%,(53.7±1.9)%,(40.8±2.7)%,(33.6±0.9)%,与常氧对照组(100.0±0)%比较,细胞存活率明显降低,差异具有统计学意义(P<0.01).染料木黄酮后处理组(100μmol/L)细胞存活率分别为(80.5±1.3)%,(72.7±1.5)%,(71.3±1.2)%,(55.1±1.0)%,与缺氧/复氧组比较,细胞存活率明显升高,差异具有统计学意义(P<0.01).荧光染色显示,缺氧/复氧组可见较多核致密浓染的凋亡细胞,而染料木黄酮后处理组凋亡细胞明显减少.结论:染料木黄酮后处理通过抑制细胞凋亡对人胃黏膜上皮细胞缺氧/复氧损伤产生一定的保护作用.AIM： To investigate the effect of genistein postconditioning on hypoxia/reoxygenation injury in human gastric epithelial cells. METHODS： Firstly, we established the model of hypoxia/reoxygenation injury using human gastric epithelial cell line （ GES-1 ）. All the cells in the present study were divided randomly into 4 groups ： normal control group （ Normal ） , hypoxia/ reoxygenation group （ H/R ）, genistein postconditioning group （Geni-PostC） and DMSO vehicle posteonditioning group （VehicPostC）. MTT assay was used to test cell livability of each group after reoxygenation （2,4,8,16 h）. Hoeehst 33258 fluoroehrome staining was employed to observe cell apoptosis in the model of H/ R （2 h/4 h）. RESULTS： MTT assay showed that the cell liva- bilityofH/Rwas （64.1±1.7）% at2h, （53.7±1.9）% at4 h, （40.8±2.7）% at8 h and （33.6±0.9）% at 16 h. Com- pared to Normal （100.0±0）%, the cell livability of H/R was significantly decreased （ P 〈 0.01 ）. The livability of Geni-PostC （100 μmol/L） was （80.5 ±1.3）% at 2 h, （72.7 ± 1.5）% at 4h, （71.3±1.2）% at8 h and （55.1 ±1.0）% at 16 h. In comparison with H/R at the same time point, the livability of Geni-PostC was significantly increased （ P 〈 0. 01 ）. Hoeehst 33258 fluorochrome staining showed that there were many apoptotic cells in H/R but few in Geni-PostC. CONCLUSION： Genistein postconditioning may have a protective effect on hypoxia/reoxygenation injury in human gastric epithelial cells by inhibiting apoptosis.

关 键 词：染料木黄酮 药物后处理 缺氧/复氧 

分 类 号：R573.39[医药卫生—消化系统]