β受体阻断剂卡维地洛抗肝纤维化的作用  

The effects of Carvedilol on CCl4-induced hepatic fibrosis of rats

作　　者：樊晓明[1] 田培营[1] 俞华芳[1] 史继敏[2] 范卫[2] 

机构地区：[1]复旦大学附属金山医院消化内科,上海200540 [2]复旦大学附属金山医院中心实验室,上海200540

出　　处：《胃肠病学和肝病学杂志》2008年第12期1033-1036,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：上海市卫生局资助项目(034102)

摘　　要：目的研究非选择性β受体阻断剂和α-1受体阻滞剂卡维地洛对抗大鼠肝纤维化的作用及机理。方法40只SD大鼠分为4组,分别为正常对照组、肝纤维化组、单用卡维地洛组、卡维地洛治疗组,分别测定各组的肝功能[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白/球蛋白(A/G)],肝纤维化血清指标(PC4、HA、CN),肝纤维化组织学指标(HE染色,Masson染色),SP免疫组织化学法测定肝组织α平滑肌肌动蛋白(α-SMA)和RT-PCR法测定肝组织转化生长因子β1 mRNA(TGF-β1 mR-NA)。结果与造模组相比,卡维地洛改善了肝功能,血清肝纤维化指标和病理学检查指标都有所下降。结论卡维地洛能够减轻CCl4致大鼠的肝纤维化。Objective To investigate the effects of nonselective beta-adrenoceptor and selective alphal-adrenoceptor blocker Carvedilol on CCl4-induced hepatic fibrosis of rats. Methods Forty SD rats were randomly divided into four groups, ten in every group. The model group was induced by CCl4 2 times per week, the control group was given with oil in the same way. The third group was fed with Carvedilol 4 mg · kg^-1·d^-1. The fourth group was treated with Carvedilol in the same way and induced by CC14 like the model group. Parameters such as AST, ALT, ALB, PC4, CN and HA were measured. Assessments of hepatic fibrosis by HE stained, Masson stained were made in image analysis. The expression of α-SMA in hepatic tissue was detected with immunohistochemical methods. Also TGF-β1 mRNA of hepatic tissue was detected with RT-PCR. Results Compared with the model group, the fibrosis assessments of the carvedilol treated group （PC4, CN, HA in serum and hepatic HE stained and collagen fiber stained） were decreased. The expres- sions of TGF-β1 mRNA were also decreased in Carvedilol treated group than in the model group. Conclusion Carvedilol can alleviate liver fibrosis of rats induced by CCl4.

关 键 词：肝纤维化 卡维地洛 Α-SMA TGF—β1 

分 类 号：R575[医药卫生—消化系统]