衣康酸高温高产菌株的选育  

Breeding of thermostable high-yielding strain for itaconic acid production

作　　者：李霞 张希铭 徐建春 

机构地区：[1]青岛琅琊台集团股份有限公司,青岛266400

出　　处：《工业微生物》2008年第6期49-52,共4页Industrial Microbiology

摘　　要：以生产使用菌种土曲霉NLYT234为出发菌株，经紫外线、高温、氯化锂诱变处理和多次分离、筛选获得一支性能稳定的衣康酸高产突变株土曲霉NLYT-2—801，在含淀粉糖140g／L的培养基上40℃－43℃摇瓶72h，衣康酸产量92．1g/L，糖酸转化率63．18％～67．83％。经300m^3发酵罐生产验证，诱变后菌种的酸度和糖酸转化率分别比以前提高了31．1％和6％。Treated by ultraviolet ray, high temperature, lithium chloride mutagenesis and repeated segregation , a thermostable mutant of Aspergillus terreus NLYT-2-801 for high yielding itaconic acid was obtained from the original producer of Aspergillus terreus NLYT234. Then cultured in the medium with starch sugar 140g/L at 40--43℃ for 72 hours, the production by NLYT-2-801 was as high as 92. 1g/L and the rate of converting sugar to acid was 63.18% 67.83 %. The verification test in a 300m^3 fermenter, the acidity and the rate of converting sugar to acid were increased by 31.1% and 6 % respectively than before.

关 键 词：土曲霉 衣康酸 诱变育种 转化率 

分 类 号：TQ921[轻工技术与工程—发酵工程]