节杆菌M3黄嘌呤氧化酶的诱导效应研究  

Induction Effect on Xanthine Oxidase Yield from Arthrobacter M3

作　　者：王静[1] 孙艳[1] 杨海麟[1] 王武[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《生物技术》2008年第6期69-71,共3页Biotechnology

基　　金：工业生物技术教育部重点实验室基金项目资助(No.KLIB-KF200502)

摘　　要：目的:寻找高效廉价的诱导物,并研究其诱导条件,以期进一步提高节杆菌M3(ArthrobacterM3)产黄嘌呤氧化酶(xan-thine oxidase,简写为XOD)水平。方法:以产酶水平和生产成本为标准,比较了几种化合物对ArthrobacterM3的诱导产酶的效果,选出最适诱导物,并对其诱导条件进行了研究。结果:几种化合物中,次黄嘌呤的诱导效果最佳。对次黄嘌呤诱导效应的研究发现,在发酵前期加入3g/L的次黄嘌呤对产黄嘌呤氧化酶的诱导效果最好。节杆菌M3降解次黄嘌呤的代谢产物中,尿素的存在对XOD的合成有强的抑制作用,而添加甘氨酸能促进XOD的产生。结论:在最佳条件下,次黄嘌呤诱导节杆菌M3产酶水平728.33U/L,超过了国内所报道的最高水平。Objective：In order to raise the yield of xanthine oxidase （for short, XOD） from Arthrobacter M3, a highly efficiem and low- cost inducer was sought, the optimal induced condition was studied. Method： Based upon the xanthine oxidase activity and the production cost, several samples of chemicals were tested to compare their inducing effects on Arthrobacter M3. The optimal inducer was checked out, and its induced condition was investigated. Result： Among these several compounds, hypoxanthine was found to be the optimal inducer. The study of hypoxanthine induced condition indicated the xanthine oxidase activity of mutant M_3 would be the highest when 3g/L hypoxanthine was added to the culture in the prophase of the fermentation. Among the metabolite decomposed hypoxanthine by Arthrobacter M3, urea could repress the production of XOD dramatically, while glycin accelerated the production of XOD. Conclusion： Under the optimal condition, the XOD activity induced by hypoxanthine reached 728.33U/L, which exceeded the highest- level from the domestic report.

关 键 词：节杆菌 黄嘌呤氧化酶 诱导 次黄嘌呤 

分 类 号：Q935[生物学—微生物学]