子宫内膜癌VEGF-D、MLD的表达及其与淋巴结转移的关系  

Relationship between Expression of VEGF-D and MLD and Lymph Node Metastasis in Endometrial Carcinoma

作　　者：董莉[1] 黎卫平[1] 闫文静[1] 吴素香[1] 

机构地区：[1]兰州大学医学院病理学研究所,730000

出　　处：《肿瘤防治研究》2008年第12期885-887,891,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨子宫内膜癌组织中血管内皮生长因子D(VEGF-D)和微淋巴管密度(MLD)的表达与淋巴结转移的关系。方法选取子宫内膜癌标本53例,正常子宫内膜标本20例,以淋巴管特异性标记物D2-40标记淋巴管并计数MLD,运用免疫组织化学技术检测VEGF-D和MLD在子宫内膜癌、正常内膜组织中的表达。结果(1)VEGF-D和MLD在子宫内膜癌组织中的阳性表达率及密度值(39/53,23.54±7.84)均显著高于正常内膜组织(5/20,10.48±1.62)(P<0.05);两者在淋巴结转移组的阳性表达率及密度值(33/38,32.05±3.27)也明显高于无淋巴结转移组(6/15,20.23±4.57)(P<0.05);VEGF-D的表达在不同年龄、不同组织分化程度、不同浸润深度之间差异均无统计学意义(P>0.05),在临床分期的表达中差异有统计学意义(P<0.05)。(2)子宫内膜癌VEGF-D的表达与MLD存在相关性,相关系数rs=0.398(P<0.05),VEGF-D阳性组MLD(31.17±2.82)较VEGF-D阴性组MLD(16.75±3.63)显著增高(P<0.05)。结论VEGF-D在子宫内膜癌中呈高表达且与微淋巴管密度(MLD)呈正相关,其可能通过诱导淋巴管生成促进子宫内膜癌淋巴结转移,可作为指导子宫内膜癌诊治及判断预后的一个重要指标。Objective To analyze the relationship between expression of vascular endothelial growth factor D （VEGF-D） and the mierolymphatic density （MLD） and lymph node metastasis in endometrial carcinoma. Methods Immunohistochemieal method was used to detect VEGF-D and MLD in 53 cases of endometrial carcinoma and 20 cases of normal endometria. Lymphatic vessels were marked by D2-40 and the microlymphatic density （MLD） was counted by Masakazu＇s method. Results （1） The positive expression rate of VEGF-D and MLD in the endometrial carcinoma tissue （39/53,23.54 ± 7. 84） were much higher than that in normal endometria tissue （5/20,10. 48 ± 1.62） （P〈0. 05） ; the positive expression rate of VEGF-D and MLD in lymph-node-positive group （33/38,32. 05 ± 3. 27） were much higher than that in lymph-node-negative group （6/15,20. 23 ± 4. 57） （P〈0. 05） ; the positive expression rate of VEGF-D in endometrial carcinoma had no correlation with age, differentiation and depth of invasion （P〉0. 05 respectively）, hut was significantly related to clinical stage （P〈0. 05）. （2） The positive expression of VEGF-D had correlation with MLD （rs = 0. 398, P〈0. 05）. The MLD of the positive group of the VEGF-D （31.17 ± 2. 82） was higher than in the negative group （16. 75 ±3.63）（P〈0. 05）. Conclusion The positive expression of VEGF-D in endometrial carcinoma has significantly correlation with MLD and it may induce lymphangiogenesis and promote the lymph node metastasis of endometrial carcinoma. The VEGF-D may be used as a valuable index in evaluating diagnosis, treatment and prognosis of endometrial carcinoma.

关 键 词：子宫内膜癌 VEGF-D MLD淋巴结转移 

分 类 号：R737.33[医药卫生—肿瘤]