Survivin靶向siRNA抑制大肠癌细胞增殖的作用  

INHIBITING EFFECT OF THE SURVIVIN-TARGETTING siRNA RECOMBINANT EXPRESSION VECTOR ON HUMAN COLON CANCER CELL PROLIFERATION

作　　者：蔡明[1] 王国斌[1] 陶凯雄[1] 蔡昌学[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,武汉430022 [2]华中科技大学同济医学院病原生物系微生物学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2008年第6期519-522,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家863高技术研究发展计划资助项目(2001AA218051);湖北省科技攻关计划资助项目(2005A304B09)

摘　　要：目的构建survivin靶向si RNA重组表达载体,研究其对人大肠癌细胞生长的抑制作用。方法构建sur-vivin靶向si RNA并转染结肠癌细胞,通过RT-PCR和Western blot方法检测survivin的表达,采用MTT法检测si RNA对细胞增殖的抑制作用。结果经酶切鉴定和测序结果证实survivin靶向si RNA重组表达载体构建成功,它对大肠癌细胞survivin mRNA和蛋白的表达抑制率分别为36.33%和44.65%,肿瘤细胞的生长受到明显抑制。结论survivin靶向si R-NA构建成功并能显著抑制survivin基因的表达。Objective To construct the siRNA recombinant expression vector targetting survivin gene and to research its inhibiting effect on human colon cancer cell proliferation. Methods SiRNA targetting＇survivin gene was constructed and transfected into human colon cancer cells. The effect of siRNA was detected by RT-PCR, Western blot and MTT reduction assay. Results It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targetting survivin gene was constructed successfully. Inhibition ratio of survivin siRNA mRNA and protein levels was 36. 33%and 44. 65% respectively, and the growth Of cancer cells was inhibited. Conclusion The siRNA targetting survivin gene has been constructed successfully and can significantly inhibite the expression of survivin gene in human colon cancer cells.

关 键 词：SURVIVIN RNA干扰 基因治疗 

分 类 号：R730.5[医药卫生—肿瘤]