mB7-1-GPI融合蛋白对小鼠肺癌的治疗作用研究  

Antitumor effect of mB71-GPI fusion protein on mouse lung cancer

作　　者：朱广[1] 肖刚明[1] 易平勇[1] 周石林[1] 

机构地区：[1]湖南省肿瘤医院胸心外科,湖南长沙410006

出　　处：《世界肿瘤杂志》2008年第4期261-264,共4页Tumour Journal of the World

摘　　要：目的 将mB7-1-GPI融合蛋白锚定于小鼠肺癌细胞膜上，制备肿瘤细胞疫苗，研究该瘤苗的抗肿瘤作用。方法将mB7-1-GPI融合蛋白锚定于小鼠肺癌细胞膜上，制备肿瘤细胞疫苗。采用流式细胞术检测该蛋白的细胞膜锚定作用。建立C57BL小鼠的肺癌模型，观察用mB7-1-GPI融合蛋白制备的肿瘤疫苗对荷瘤小鼠的免疫治疗作用。结果mB7-1-GPI融合蛋白能够锚定在小鼠肺癌细胞膜上，能有效刺激小鼠脾细胞增殖和分泌IL-2和IFN-γ。融合蛋白制备的肿瘤疫苗能够显著抑制荷瘤小鼠肿瘤的生长，并延长其生存期。结论mB7-1-GPI融合蛋白制备的肿瘤疫苗具有较强的抗肿瘤作用，有可能作为一种有效的新型疫苗用于肿瘤的治疗和预防。Objective To investigate its antitumor effects of mB7-1-GPI fusion protein on mouse lung cancer. Methods mB7-1-GPI on tumor cell membrane was incorporate and mB7-1-GPI-anchoring tumor vaccine was prepared. The antitumor immunity induced by the prepared mB7-1-GPI-anchored tumor cell vaccine in tumor-bearing mice was observed. Results mB7-1-GPI fusion protein was stably anchored onto the surface of Lewis tumor cells, and the surface protein showed its biological activities by stimulating lymphocyte proliferation and inducing lymphocytes to release IL-2 and IFN-γ, in vitro. The tumor cell vaccine prepared from Lewis cells coated with mB7-1-GPI exhibited antitumor effect. Conclusions The tumor vaccine prepared with mB7-1-GPI fusion protein may significantly inhibit the tumor growth in Lewis bearing mice. It represents a useful new strategy for attaching immunological factor onto tumor cell surfaces without genetic manipulation.

关 键 词：融合蛋白 疫苗 肿瘤 免疫治疗 肺癌 

分 类 号：R730.5[医药卫生—肿瘤]