互隔交链孢霉素对NIH/3T3细胞毒性的作用  

Cytotoxicity of altenuene on NIH/3T3 cells

作　　者：刘康栋[1] 马艳英[1] 赵继敏[1] 王瑾瑾[1] 杨洪艳[1] 黄幼田[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理教研室,河南郑州450052

出　　处：《毒理学杂志》2008年第6期409-412,共4页Journal of Toxicology

基　　金：国家自然科学基金项目(30471952);河南省自然科学基金项目(2007310020)

摘　　要：目的研究互隔交链孢霉素(altenuene,ALT)对NIH/3T3细胞的毒性作用,为互隔交链孢霉的致癌机制提供理论依据。方法以互隔交链孢酚(alternariol,AOH)为阳性对照,采用形态学观察,噻唑蓝法,生长曲线,流式细胞术(FCM)等方法检测不同浓度的ALT对NIH/3T3细胞生长的影响及细胞周期的改变。结果ALT对NIH/3T3细胞的半数抑制率为76.4、50和100μmol/L的ALT染毒24 h可使NIH/3T3细胞生长曲线明显下降,并有明显的形态学改变;以10.0、20.0和50.0μmol/L的ALT染毒24 h后,与对照组比较,G2/M期细胞比例增加,且差异有统计学意义(P<0.05)。结论ALT对NIH/3T3细胞有毒性作用,可抑制细胞增殖并诱导G2/M期细胞阻滞,其细胞抑制作用可能与细胞周期阻滞有关。Objective To study the toxic effects of ahenuene （ALT） on NIH/3T3 cells , and provide some evidence to the mechanism of carcinogenesis of alternaria. Methods Taking the ahernariol （AOH） for the positive control, the effects of ALT on growth curve and cell cycle of NIH/3T3 cells were detected by the alteration of the cell morphology, methyl thiazolyl tetrazolium（MTT） assay, growth curve, flow cytometric assay（FCM）.Results The median inhibit dose（IC50 ） of NIH/3T3 cells was 76.4 μmol/L; The growth curve of NIH/3T3 cell was decreased obviously, after treated with 50 μmol/L and 100 μmol/L ALT for 24 h, and with morphological alterations in a dose-dependent manner; Compared with the control group the percent of G2/M phase cells was increased after treated with 10.0,20.0,50.0 μmol/L ALT for 24 h （ P 〈 0.05 ）. Conclusion ALT had the cytotoxic effects on NIH/3T3 cells, and inhibited cell proliferation and induced cell cycle arrest in G2/M phase. The mechanism may be related to the arrest of cell cycle.

关 键 词：互隔交链孢霉素 NIH/3T3细胞 细胞毒性 生长曲线 细胞周期 

分 类 号：R730.231[医药卫生—肿瘤]