TNP-470联合CTX对小鼠LA795肺腺癌皮下移植瘤生长的影响  

Inhibitory Effects of TNP-470 in Combination with CTX on Growth of Lung Adenocarcinoma LA795 in Mice

作　　者：王小华[1] 张平[2] 李国亮[1] 张忠山[1] 王铮[3] 

机构地区：[1]湘南学院附属医院肿瘤科,湖南郴州423000 [2]南华大学护理学院,湖南衡阳421001 [3]中国医学科学院肿瘤研究所药物检测中心,北京100021

出　　处：《湘南学院学报（医学版）》2008年第4期9-12,共4页Journal of Xiangnan University（Medical Sciences）

基　　金：湖南省卫生厅科研基金资助(2003090)

摘　　要：目的研究血管生成抑制剂TNP-470联合CTX对小鼠L795肺腺癌生长的抑制作用。方法将40只接种高转移性的LA795肺腺癌细胞T739小鼠随机分成5组:对照组、溶剂对照组、TNP-470组、环磷酰胺(CTX)组和TNP-470+CTX组。接种4d后开始用药,用药期间,每隔1d测量皮下移植瘤体积,20d后处死全部小鼠,剥离皮下移植瘤并称重。采用免疫组化和图像分析系统定量检测皮下移植瘤中微血管密度(MVD)、血管生长因子bFGF及增殖核抗原PCNA的表达,用原位凋亡TUNEL法检测肿瘤细胞凋亡指数。结果各用药组肿瘤的生长明显受到抑制,而联合用药组抗瘤作用进一步增强,且具有协同作用。CTX组、TNP-470组和联合用药组与对照组相比都能减少bFGF表达;与CTX组相反,TNP-470组肿瘤组织中抗原PCNA的表达与对照组相比无差异显著性。TNP-470组和联合用药组肿瘤组织中的MVD与对照组相比都明显减小;而各用药组的凋亡指数比对照组都明显增高。结论TNP-470组、CTX组中LA795肺腺癌生长受到明显抑制,两者联合应用具有协同作用。Objective To study the effect of angiogenesis inhibitor TNP- 470 combination with CTX on the growth of lung adenocareinoma LA795 in mice. Methods Forty T739 mice beating high metastatic LA795 tumor cells were randomized into five groups： control group, vehicle control group, TNP - 470 group, CTX group and TNP - 470 ＋ CTX group. Different treatments were served from 4th day after transplantation and all mice were sacrificed after 20 days. The volumes of tumors were measured respectively during the therapy time, and subcutaneous tumors were processed for histological examination. Tumors microvessel density （MVD）, the expression of PCNA and bFGF were detected by immunohistochemical method with image analyse system. Apoptosis in tumors was measured by biotinyated - dUTP nick and labeling （TUNEL） method. Results In each treatment group tumor growth was suppressed significantly. The combination group showed significant enhancement in anti- tumor efficacy. Expression of bFGF was clearly inhibit in each treatment group, but apoptosis was enhanced,as compared with the control group. Contrary to CTX, TNP- 470 had no effects on the expression of PCNA in tumor. Tumor MVD was lower in combination group or TNP - 470 group compared to control group. Conclusion TNP - 470 can inhibit the growth of lung adenocarcinoma LA795. When combined with CTX, it may produce synergetic effect.

关 键 词：TNP-470 LA795 凋亡 血管生成抑制剂 

分 类 号：R734.2[医药卫生—肿瘤]