产中性纤维素酶真菌产酶条件的优化  

Optization of conditions for submerged fermentation of neutral cellulase by fungi

作　　者：曾青兰[1] 

机构地区：[1]咸宁职业技术学院生物工程系,湖北咸宁437100

出　　处：《华中师范大学学报（自然科学版）》2008年第4期612-614,共3页Journal of Central China Normal University：Natural Sciences

摘　　要：研究了碳源、氮源、接种量、培养时间、培养温度、培养基初始pH等条件对产中性纤维素酶真菌菌株瓶霉菌（Phialophora sp．z8）产酶的影响．结果表明：该菌株产中性纤维素酶的适宜碳源为1％稻草粉加1％可溶性淀粉，最适氮源为0．2％的（NH4）2SO4加0．1％的牛肉膏，接种量为5％，培养时间为72h，培养温度为28℃，培养基初始pH为6～7．在最适宜的条件下，培养液中内切葡聚糖酶活力可达到2．95IU／mL，而天然纤维素酶活力和滤纸酶活力却很低。Factors including carbon source , nitrogen source ,inoculating quantities ,incubating time ,initial temperature ,initial pH et al affecting the production of cellulase have been studied. The results showed that the best carbon source wasl%straw powder and 1% soluble starch, nitrogen source was 0.2 % （NH4）2 SO4 and 0.1%peptone,inoculating volume was 5%,incubating time was 72 h ,initial temperature was 28℃ and initial pH of culture medium was 6～7. Under the optimal condition the activity of cellulase （CMCase） reached 2.95 IU/mL ,natural cellulase and FPA were very low.

关 键 词：中性纤维素酶 PHIALOPHORA sp.z8 酶活力 优化 

分 类 号：Q939.3[生物学—微生物学]