蓖麻蚕不同组织器官中胰蛋白酶抑制剂的种类与分析  

Investigation of Trypsin Inhibitor Types From Different Tissues of the Eri-silkworm,Philosamia cynthia ricini

作　　者：王凌燕[1] 钟晓武[1] 张艳[1] 莫现会 谭祥[1] 赵萍[1] 

机构地区：[1]西南大学生物技术学院,农业部蚕桑学重点开放实验室,重庆400716 [2]广西壮族自治区蚕业技术推广总站,南宁530007

出　　处：《蚕业科学》2008年第4期761-766,共6页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划“973”项目(编号2005CB12-1000)

摘　　要：胰蛋白酶抑制剂（TI）是鳞翅目昆虫体内广泛存在的一种蛋白酶抑制剂，主要功能是抑制胰蛋白酶的降解。采用非变性聚丙烯酰胺凝胶电泳和胰蛋白酶抑制剂活性染色，对18个蓖麻蚕品种5龄第5天的血液及其它组织中TI种类的分布进行了调查。经碱性聚丙烯酰胺凝胶电泳，在血液中共检测到8种TIs，分别命名为ETI-1～ETI-8，品种间存在多态性分布；在头和脂肪体中分别检测到1种TI；在体壁中检测到3种TIs。经酸性聚丙烯酰胺凝胶电泳，在血液中共检测到4种TIs，分别命名为ETI-b1、ETI-b2、ETI-b3和ETI-b4，品种间种类分布无差异；在血液中检测到的TI包含了在头、脂肪体和体壁中检测到的TI种类。Trypsin inhibitors （TIs） present widely in bodies of the Lepidoptera insects, which play important roles in inhibiting proteolysis of trypsin. By using method of native-polyacrylamide gel electrophoresis followed by active staining, we investigated the polymorphism of TIs in hemolymph and major tissues at 5th d of 5th instar of 18 varieties of eri-silkworm, Philosamia cynthia ricini. In the result of the alkaline native-polyacrylamide gel electrophoresis, there are 8 TIs in hemolymph, named as ETI-1～ ETI-8, and a little difference among different varieties. Only 1 TI was detected in head and fat body, respectively, while 3 TIs were detected in body wall. In the result of the acidity native-polyacrylamide gel electrophoresis, 4 TIs were detected in hemolymph, which were named as ETI-bl, ETI-b2, ETI-b3, and ETI-b4, respectively. The distribution of TIs in hemolymph among 18 eri-silkworm varieties was same. TIs in hemolymph included TIs detected in head, fat body, and body wall.

关 键 词：蓖麻蚕 胰蛋白酶抑制剂 分布 品种 组织 非变性聚丙烯酰胺凝胶电泳 

分 类 号：S885.2[农业科学—特种经济动物饲养]