胶质瘤细胞增殖活性和凋亡与临床病理特征的相关性研究  

Relationship between Levels of Proliferation and Apoptosis with Clinicopathological Characteristics in Brain Gliomas

作　　者：曹慧玲[1] 孔霞[2] 崔文[1] 高继发[1] 王旭[1] 田德明[1] 

机构地区：[1]济宁医学院病理学教研室 [2]济宁医学院附属第一人民医院

出　　处：《济宁医学院学报》2008年第4期275-278,共4页Journal of Jining Medical University

摘　　要：目的探讨胶质瘤中细胞增殖和凋亡的关系及其与临床病理特征的相关性。方法采用常规免疫组化技术和TUNEL技术分别检测96例人脑胶质瘤中瘤细胞增殖活性(PCNA蛋白的表达)和细胞凋亡的改变。结果96例胶质瘤中Ⅱ、Ⅲ、Ⅳ级肿瘤的PCNA标记指数(PCNA LI)分别为26.79±3.45%、44.32±5.83%、83.31±13.67%,显著高于正常脑组织(2.67±1.31%)(P<0.05),并且随着肿瘤分级的升高而递增。在Ⅱ、Ⅲ、Ⅳ级肿瘤中凋亡指数(AI)分别为18.92±7.21,10.18±4.25,5.25±1.93,并随分级的升高而递减,Ⅱ级和Ⅲ、Ⅳ级肿瘤中AI有显著差异,Ⅲ、Ⅳ级间无差异。PCNA LI与AI呈显著负相关(P<0.05)。各级别胶质瘤中PCNA LI/AI随着病理分级的升高而增大,呈显著正相关性(P<0.01)。结论胶质瘤的发生和发展与瘤细胞增殖活性的增高和细胞凋亡的抑制密切相关,胶质瘤PCNA LI/AI比PCNA LI或AI单独检测更能反映胶质瘤的病理特征及恶性行为。Objective To investigate the levels of proliferation and apoptosis of brain glioma ceils and their relationship to the clinicopathological characteristics. Methods 96 cases of brain gliomas were chosen, and the protein expression of proliferating cell nuclear antigen （PCNA） and apoptosis of tumor ceils were investigated respectively by immunohistochemistry method and TdT mediated dUTP label index （PCNA LI） and the apoptosis index （AI） of brain nick - end labeling （TUNEL） method. The PCNA gliomas were calculated. Results The PCNA LI in gliomas of grade Ⅱ ,grade Ⅲ and IV were 26.79 ± 3.45% ,44.32 ±5.83% and 83.31 ± 13.67% ,respectively, and higher than that in normal brain tissue. The PCNA LI increased progressively along with grade of gliomas steping up, but AI decreased （ P 〈 0.05）. AI of grade Ⅱ and Ⅲ tumors were significantly different （ P 〈 0.05）, but there was not any difference between grade Ⅲ and Ⅳ. The negative correlation was suggested between PCNA LI and AI（ P 〈0.05）. PCNA LI/AI increased progressively along with grade of gliomas steping up. There was an positive correlation between them. Conclusion The overexpression of PCNA protein and inhibition apoptosis may play an important role in the genesis and development of gliomas. Detecting PCNA LI/AI was more significant than doing PCNA LI or AI respectively in determing clinicopathlogic features and malignant behaviors.

关 键 词：胶质瘤 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R739.41[医药卫生—临床医学]