乳腺癌组织中CDK4 mRNA的表达及意义  

Expression and significance of CDK4 mRNA expression in breast cancer tissues

作　　者：陆辉[1] 王水[1] 王锋[1] 

机构地区：[1]南京医科大学第一附属医院普通外科,210029

出　　处：《江苏医药》2008年第10期1008-1009,共2页Jiangsu Medical Journal

基　　金：江苏省卫生厅重大科研资助项目(135-48)

摘　　要：目的探讨乳腺癌组织中CDK4 mRNA的表达及临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)法检测45例乳腺癌组织、癌旁组织及25例正常乳腺组织中CDK4 mRNA的表达。结果CDK4 mRNA阳性表达率在乳癌组织、癌旁组织及正常乳腺组织中分别为51.11%、15.56%和0,与肿瘤组织分级、淋巴结转移显著相关,与孕激素受体、雌激素受体无相关性。结论CDK4 mRNA与乳腺癌的发生、发展及淋巴结转移密切相关,可作为判断肿瘤生物学行为指标之一。Objective To explore the expression and significance of CDK4 mRNA in breast carcinomas. Methods The expressions of CDK4 mRNA of breast cancer tissues and tumor-adjacent tissues in 45 patients and normal breast tissues in 25 cotrols were detected by reverse transcriptase polymerase chain reaction （RT-PCR）. Results The positive rates of CDK4 mRNA expression in breast cancer tissues ,tumor-adjacent breast tissues and normal breast tissues were 51.11%, 15.56% and 0, respectively （P〈0.05）. The expression level of CDK4 mRNA in breast carcinoma was significantly associated with histological degree, lymph node metastases （P〈 0.01 ）, and little associated with ER and PR status （P〉0.05）. Conclusion The CDK4 mRNA plays a key role in the oncogenesis, development and lymph node metastases of breast cancer and could be an useful mariner for assessing the malignant level of breast carcinoma and its clinical prognosis.

关 键 词：CDK4 MRNA 乳腺癌 雌激素受体 肿瘤生物学行为 

分 类 号：R737[医药卫生—肿瘤]