干扰素诱导的跨膜蛋白-1对诊断结直肠癌的临床价值  

Detection of interferon-induced transmembrane-1 gene expression for clinical diagnosis of colorectal cancer

作　　者：刘宇虎[1] 柳娟[1] 郭健[1] 游志坚[1] 王在国[1] 钟东[2] 杨兴龙[2] 张振书[2] 肖冰[2] 郭文英[1] 

机构地区：[1]东莞市人民医院消化科,广东东莞523018 [2]南方医科大学南方医院消化病研究所,广东广州510515

出　　处：《南方医科大学学报》2008年第11期1950-1953,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30171053);广东省医学科研项目(B2005089);东莞市科技计划项目(B200501)

摘　　要：目的探讨干扰素诱导的跨膜蛋白-1(IFITM1)基因在肿瘤组织中的表达,血清抗IFITM1的抗体反应以及对临床诊断结直肠癌的意义。方法应用半定量RT-PCR方法检测IFITM1基因mRNA在正常大肠粘膜、结直肠癌组织、大肠炎性息肉、腺瘤性息肉、胃癌、食管癌和肝癌组织中的表达。采用Western印迹法检测患者血清抗IFITM1的抗体反应。分析有IFITM1基因表达癌肿的病理特点。结果结直肠癌组织中IFITM1基因mRNA表达率为47.4%(18/38),显著高于大肠腺瘤性息肉的15%(3/20)和胃癌组织的4.8%(1/21)(P<0.05),而正常大肠粘膜、大肠炎性息肉、食管癌和肝癌组织均无表达。结直肠癌组织IFITM1基因mRNA呈强表达,其表达水平(0.8048±0.2273)显著高于大肠腺瘤性息肉(0.4447±0.0989)(P<0.001)。结直肠癌血清相应的抗体反应率为36.8%(14/38),明显高于胃癌的9.5%(2/21)(P<0.05),而在食管癌、肝癌、大肠炎性息肉和大肠腺瘤性息肉以及健康人血清则未检测出相应的抗体反应。有IFITM1基因表达的结直肠癌多数直径大于5cm,侵及浆膜,有淋巴结和远处转移,多属DukesC期和D期,以高分化腺癌为主。结论IFITM1基因可能在结直肠癌的发生、发展和转移过程中起重要作用,有望成为临床诊断结直肠癌的一个良好标志物。Objective To investigate the expression of the interferon-induced transmembrane-1 （IFITM1） gene in colorectal cancer （CRC） tissue and the serum anti-IFITM1 antibody responses of the patients and assess their value in clinical diagnosis of CRC. Methods Semi-quantitative RT-PCR was performed to detect IFITM1 mRNA expression in the specimens of normal colonic mucosa, CRC tissue, inflammatory polyps, adenomatous polyps, gastric cancer, esophageal carcinoma and liver cancer tissues. Serum samples were Collected from the patients to detect anti-IFITM1 antibody responses using Western blotting. The clinicopathological features of the carcinoma expressing IFITM1 gene were analyzed. Results IFITM1 mRNA was expressed in 47.4 % （18/38） of the CRC specimens, a rate significantly higher than that in adenomatous polyps [15% （3/20）] and gastric cancer [4.8% （1/21）]; no Obvious IFITM1 expression was found in normal colonic mucosa, inflammatory polyp, esophageal carcinoma or liver cancer tissues （P〈0.001 or P〈0.05）. IFITM1 mRNA was strongly expressed in CRC at the expression level of 0.8048±0.2273, which was significantly higher than that in adenomatous polyps （0.4447±0.0989, P〈0.001）. No anti-IFITM1 antibody response was detected in healthy human sera, but in the CRC patients, the serum antibody response was detected at the rate of 36.8% （14/38）, significantly higher than the rate of 9.5% （2/21） in gastric cancer （P〈0.05）. No antibody response was detected in esophageal carcinoma, liver cancer, inflammatory polyp or adenomatous polyps. Most of the IFITM1-expressing CRC had a diameter exceeding 5 cm, often invading the serous membrane with metastasis to the lymph nodes and the distant organs; these tumors were identified mostly as well-differentiated adenocarcinoma in Dukes stage C or D. Conclusion IFITM1 gene may play an important role in the pathogenesis, development and metastasis of CRC, and may serve as a potential biomarker for clinical diagnosis of CRC.

关 键 词：结直肠癌 干扰素诱导的跨膜蛋白-1 基因表达 抗体反应 

分 类 号：R735.3[医药卫生—肿瘤]