骨肉瘤荷瘤裸鼠模型两种不同制作方法的比较  

Comparison of two different methods for establishing nude mouse model bearing osteosarcoma

作　　者：管明强[1] 史占军[1] 李鹏[1] 杨澜波[1] 

机构地区：[1]南方医科大学南方医院脊柱骨科,广东广州510515

出　　处：《南方医科大学学报》2008年第11期1960-1963,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30371450);广东省自然科学基金(32948;984078)

摘　　要：目的比较腋下移植与原位移植两种骨肉瘤荷瘤裸鼠模型制作方法在成瘤率、成瘤时间、瘤体生长变化,特别是肺部转移情况的差别。方法将骨肉瘤细胞分别接种到裸鼠左侧腋下及裸鼠股骨髓腔,6周后处死裸鼠,肉眼及光镜下观察两组肿瘤的肺部转移情况,比较两组肿瘤肺部转移率及转移灶的不同特点。结果与原位接种方式相比,腋下接种方式操作简单、成瘤快,肿瘤组织表面容易较早发生摩擦破损(P<0.05),肿瘤内部也较早发生缺血坏死现象(P<0.05),在相同接种时间下,腋下接种组裸鼠肺部肿瘤转移率低(P<0.05),发生肺部转移时,转移灶也较为稀疏(P<0.05),体积较小。原位移植则能够很好地模拟骨肉瘤本来的生长环境,在接种时间时相同,具有肿瘤缺血坏死现象少、肺部转移率高、转移灶密集、转移灶体积较大等优点,但该方法操作步骤相对复杂。结论两种裸鼠模型制作方法均具有较高的成瘤率,但在制作骨肉瘤肺部转移模型方面原位移植裸鼠模型制作方法明显优于腋下移植裸鼠模型制作方法,且原位移植形成的肿瘤表面摩擦破损及内部缺血的坏死发生也相对较晚。Objective To compare two different methods, namely orthotopic implantation and armpit implantation, for establishing nude mouse model beating osteosarcoma in light of the tumor formation rate and time, tumor growth changes and lung metastasis. Methods Osteosarcoma cells were inoculated in the left armpit or the femoral medullary canal of nude mice, and the tumor growth was observed 6 weeks later. The differences in the pulmonary metastasis were examined macroscopically and microscopically. Results Armpit implantation allowed easy operation and fast tumor growth, but tumors often sustained damages by frictions on the surface （P〈0.05）, and avascular necrosis occurred earlier in the tumors （P〈0.05）. With this approach, the pulmonary metastasis rate was only 40% （P〈0.05）, and the metastases in the lungs were sparser （P〈0.05） and small. Orthotopic transplantation could well simulate the original growth environment of osteosarcoma, and resulted in milder avascular necrosis and greater pulmonary metastasis rate （100%） with larger and denser metastatic foci. But orthotopic transplantation required more complicated operation for establishing osteosarcoma-bearing models. Conclusion The two methods both result in high tumor growth rate, but orthotopic transplantation is superior in inducing pulmonary metastasis of osteosarcoma and reducing friction injury of the tumor and avascular necrosis in the tumor.

关 键 词：骨肉瘤 裸鼠 原位移植 腋下移植 

分 类 号：R738.1[医药卫生—肿瘤]