食管鳞癌HPV感染与病毒致癌基因E6的关系  

Relationship beween human papiloma virus DNA and E6 protein in esophageal squamous cell carcinoma

作　　者：邹赛英[1,2] 刘旭明[1,2] 唐新萍[1,2] 司静懿 

机构地区：[1]兰州军区乌鲁木齐总医院病理科 [2]中国协和医科大学基础医学研究所生物物理室

出　　处：《临床与实验病理学杂志》1998年第1期50-52,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的：探讨人乳头瘤病毒（ＨＰＶ）与食管鳞状细胞癌的关系。方法：采用多重引物多聚酶链反应（ＰＣＲ）和免疫组化技术，对１０４例食管鳞癌进行ＨＰＶＤＮＡ和病毒癌基因Ｅ６蛋白检测。结果：ＨＰＶＤＮＡ阳性者占５０．９６％（５３／１０４），其中ＨＰＶ１６型ＤＮＡ４９．０６％（２６／５３），ＨＰＶ１８型ＤＮＡ５．６％（３／５３），ＨＰＶ６／１１ＤＮＡ７．５％（４／５３）；两个或三个类型的混合感染占３７．７３％（２０／５３）。Ｅ６蛋白阳性５６例（５３．８４％），以低分化鳞癌表达强度最高。结论：提示新疆地区食管鳞状细胞癌中ＨＰＶ有较高的感染率，ＨＰＶ１６。Purpose To study the relationship between human papiloma virus DNA and E6 protein in esoghageal squamous cell carcinoma. Methods A total of 104 human esophageal squamous cell carcinoma specomens were studied for HPV by multiple PCR and E6 early protein of HPV16/18 by immunohistochemistry. Results Fiftythree (5096%) of the 104 cases were positive for HPV DNA. HPV16 DNA was found in 26(4906%) of the 53 patients, HPV18 DNA in 3(56%) and 6/11 DNA in 4(75%). But HPV 18 or 6/11 and 16 and all three mixpositive in 20(3773%) were observed. E6 protein in nucleus were present in 56(5384%) cases. A highly significant correlation between the presece of HPV and E6 was found. Conclusions The results suggest that E6 early genes of HPV16 DNA may be possible etioligical agent in esophagea carcinoma.

关 键 词：食管肿瘤 鳞癌 人乳头瘤病毒 E6基因 致癌基因 

分 类 号：R735.102[医药卫生—肿瘤]