γ射线照射大鼠肝细胞内线粒体降解过程的酸性磷酸酶细胞化学观察  

Acid phosphatase cytochemical observation of mitochondrial degradation in rat hepatocytes after γ-irradiation

作　　者：乔东访[1] 刘连璞[1] 朴英杰[1] 

机构地区：[1]第一军医大学中心实验室,广州510515

出　　处：《第一军医大学学报》1998年第1期1-3,共3页Journal of First Military Medical University

基　　金：国家自然科学基金!39470347

摘　　要：采用电镜酶细胞化学（AcP酶）方法观察了γ射线照射后大鼠肝细胞内自噬体的一系列变化特征，特别是线粒体的降解，及此过程中自噬体的形成和自噬体与溶酶体之间的相互关系。结果显示，照射后大鼠肝细胞产生大量自噬体，受损线粒体被自噬后，溶酶体参与其降解过程。By using enzyme-cytochemical method for acid phosphatase and transmission electron mi croscopy,the characteristics of the sequential alterations of autophagic vacuoles (AV) were observed after γ-irradiation in rat hepatic parenchymal ce1ls,especially the processes of mitochondrial degradation and the relationship between AV and lysosomes. The results showed that Y-irradiation caused high frequency of AV formation,and lysosomes participated in the degradation of AV.

关 键 词：Γ射线 线粒体降解 酶学 肝细胞 大鼠 

分 类 号：R818.74[医药卫生—放射医学] Q345.2[医药卫生—临床医学]