myc和ras癌基因共整合转基因小鼠的建立  

Establishment of transgenic mice cointegrated with myc and ras oncogenes

作　　者：陈汉源[1] 陆荣华 余焱林[2] 徐国江 曾位森[1] 傅江南[3] 谢卫兵[1] 蒋霞 罗琛[1,4] 

机构地区：[1]第一军医大学细胞生物学实验室,广州510515 [2]第一军医大学细胞生物学和医学遗传学教研室,广州510515 [3]第一军医大学实验动物中心,广州510515 [4]上海细胞生物学研究所,上海200031

出　　处：《第一军医大学学报》1998年第1期52-55,共4页Journal of First Military Medical University

基　　金：全军医药卫生科研基金!1594;广东省自然科学基金!920193;国家自然科学基金!39200143

摘　　要：MMTV－H3－c－myc和MMTV－v－Ha－ras癌基因DNA混合显微注射到小鼠受精卵中，并移植到假孕母鼠输卵管内，获得出生后断奶健康小鼠95R（♀55，♂40）。经过斑点杂交显示myc正反应小鼠17只（♀12，♂5），ras正反应小鼠12只（♀7，♂5）。在全部25只癌基因正反应小鼠中，有4只为myc＋ras双重正反应，均为雌性。继续进行Soutnern印迹分析，确认myc正反应小鼠为“建成者”转基因小鼠。许多转基因小鼠发生各种肿瘤。Carcinogenesis involves a multiple-step process that likely is concerned with successive stimulation of several oncogenes. Transgenic mouse models provide the means to test directly the co-operative action of activated oncogenes in vivo. Microinjection of a mixture of two gene-constructs,including MMTV-H3-c-myc and MMTV-v-Ha-ras, into the fertilized mouseeggs was performed. These embryos were implanted into the oviduct of pseudopregnant female mice. The resulting 95 littermates were analyzed by dot hybridization. 17 myc-positive mice (♀ 12, ♂ 5) and 12 ras-positive mice (♀ 7, ♂ 5) were found.Among these mce there exist 4 myc+ras double transgenic mice (all ♀). The above results were confirmed with Southern blot method. Up to date, 8 mice carrying myc and 2 double transgenic mice bearing myc+ras develop various types of tumors.

关 键 词：转基因动物 小鼠 MYC基因 RAS基因 癌基因整合 

分 类 号：R730.231.3[医药卫生—肿瘤]