乙基4-(3-氧-1,2-苯并异硒吡咯-2-基)-α-甲基-苯乙酸盐对培养大鼠皮质神经元缺氧损伤的保护作用  

Protective effect of ethy1- 4-(3-oxo-1,2-benzisoselenazol-2-yl)-α-methylbenzeneacetate on anoxic damage to cultured rat cortical neurons

作　　者：谈冶雄[1,2] 李万亥[1,2] 陶学斌 姜远英[1,2] 陈卫平 

机构地区：[1]第二军医大学药学院中西药研究室 [2]第二军医大学药学院药理学教研室

出　　处：《第二军医大学学报》1998年第1期39-41,共3页Academic Journal of Second Military Medical University

摘　　要：目的：研究乙基４－（３－氧－１，２－苯并异硒吡咯－２－基）－α－甲基－苯乙酸盐（ＥＯＢＭＢ）对培养神经元缺氧损伤的保护作用及机制。方法：培养大鼠皮质神经元通以９５％Ｎ２＋５％ＣＯ２造成缺氧模型；体外产生的超氧阴离子和羟自由基刺激培养神经元；测定培养上清乳酸脱氢酶（ＬＤＨ）活性和硫代巴比妥酸反应活性物质（ＴＢＡＲＳ）含量；观察ＥＯＢＭＢ对超氧阴离子和羟自由基特征显色反应的影响；测定谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）拟似活性。结果：ＥＯＢＭＢ能抑制缺氧和自由基诱发的培养神经元的ＬＤＨ释放和ＴＢＡＲＳ含量的增加。体外无直接灭活超氧阴离子和羟自由基的活性，但表现一定的ＧＳＨ－Ｐｘ活性。结论：ＥＯＢＭＢ对神经元缺氧损伤具有保护作用，作用机制主要通过发挥ＧＳＨ－Ｐｘ活性，抑制自由基的脂质过氧化。Objective:To study the protective effect of ethyl- 4-(3-oxo-1,2-benzisoselenazol-2-yl)-α-methyl-benzeneacetate (EOBMB) on anoxia-induced injury to cultured rat cortical neurons. [WTHZ] Methods: [WTBZ] Cultured cortical neurons were placed under 95% N_2 and 5% CO_2 as an anoxia model. O_2 produced by hypoxanthine/xanthine oxidase and ·OH produced by vitamin C/CuSO4 stimulated cultured neurons. LDH activity and TBARS content were assayed spectrophotometrically. Effect of EOBMB on color reaction of O_2 and ·OH was evaluated. GSHPx activity of EOBMB was determined. Results: EOBMB depressed LDH efflux and increase of TBARS content in cultured neurons induced by anoxia and free radicals. In vitro, EOBMB had no direct inactive activity on O_2 and · OH while showed certain GSHPx activity. Conclusion: EOBMB has a protective effect on neuron damage indued by anoxia, which derives from depressing lipid peroxidation damage induced by free radicals as a GSHPx mimicry.

关 键 词：缺氧 皮质神经元 氧自由基 脑缺血 EOBMB 

分 类 号：R743.310.5[医药卫生—神经病学与精神病学]