鼻咽癌组织中BHRF1基因的研究  

The study of BHRF1 gene in nasopharyngeal carcinoma tissues

作　　者：黄海[1,2,3,4] 潘星华[1,2,3,4] 周水淼[1,2,3,4] 李兆基 温武[1,2,3,4] 余龙 孔宪涛[1,2,3,4] 孙喜元 傅强[1,2,3,4] 

机构地区：[1]第二军医大学长海医院耳鼻咽喉科 [2]第二军医大学基础医学部生物学教研室 [3]复旦大学遗传学研究所基因工程国家重点实验室 [4]第二军医大学长征医院临床免疫中心

出　　处：《第二军医大学学报》1998年第1期85-86,共2页Academic Journal of Second Military Medical University

基　　金：军队"九五"医药科研规划课题

摘　　要：目的：探讨鼻咽癌组织中ＥＢ病毒基因ＢＨＲＦ１的存在和表达与癌细胞分化及其患者预后间的关系。方法：利用斑点杂交技术检测鼻咽未分化癌、低分化及高分化鳞癌中ＢＨＲＦ１的存在和表达情况，并且观察患者对放疗的效果。结果：ＢＨＲＦ１的存在与表达与鼻咽癌的分化无关，但其表达可导致癌细胞对辐射敏感性的下降（Ｐ＜０．０１）。结论：ＢＨＲＦ１在鼻咽癌细胞中的表达可增强其抵抗辐射诱发细胞凋亡的能力，这在鼻咽癌诊断、治疗和预后判断上具有重要意义。Objective: In order to learn the relationship between the presence and expression of BHRF1 and differentiation of tumor cells and the patient’s prognosis. Methods: Dot blot hybridization was used to test the presence and expression of BHRF1 in the cells of undifferential, low grade, high grade nasopharyngeal carcinomas(NPC) and the efficacy of radiotherapy on the patients was observed. Results: The presence and expression of BHRF1 were unrelated with the differentiation of tumor cells, but its expression could decrease cell sensitivity to radiation(P<0.01). Conclusion: BHRF1 expression could enhance NPC cells antiapoptotic ability induced by radiation which may be important to diagnosis, treatment and prediction of NPC.

关 键 词：EB病毒基因 鼻咽肿瘤 细胞分化 

分 类 号：R739.630.2[医药卫生—肿瘤]