一株海洋细菌HZBN43的鉴定  

Preliminary Identification of a Marine Bacterial Strain HZBN43

作　　者：张婷[1] 曲凌云[2] 祝茜[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264209 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《安徽农业科学》2008年第33期14380-14381,共2页Journal of Anhui Agricultural Sciences

基　　金：近海海洋微生物资源的标准化整理整合与共享(2005D-KA21209)

摘　　要：[目的]鉴定1株分离自黄海海水的细菌菌株HZBN43。[方法]采用16S rRNA技术,对细菌样品株HZBN43进行鉴定,并对其进行系统分析。[结果]通过PCR扩增获得1 521 bp的16S rRNA序列,将其与NCBI已登陆的其他同源序列进行系统分析,确定了此株海洋细菌属于芽孢杆菌属(Bacillus)。虽然与已知种Bacillus selenatarsenatis的相似性为99.79%,但系统分析表明,置信度仅为46,可能受分析序列较短所限。要将其确定到种,尚需要其他研究佐证。[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea. [Method] Using 16S rRNA technique, a strain from Yellow Sea was preliminarily identified and analyzed. [Result] A 1521 bp fragment of 16S rRNA was amplified from the strain HZBN43; homology analysis between the yielded sequence and the 16S rRNA sequences accessed in NCBI from other strains showed that HZBN43 belonged to Bacillus ,and shared 99.79% homologue with the known species Bacillus selenatarsenatis. However, because of the incomplete sequence, the confidence level is just 46, so other corroborations are still required for grouping HZBN43 into an exact species.

关 键 词：海洋细菌HZBN43 16S RRNA 芽孢杆菌属 同源性分析 

分 类 号：S188[农业科学—农业基础科学]