Muc-1和MDR3在胆囊结石形成过程中的作用  

Roles of MUC-1 and MDR3 in the formation of cholesterol calculus

作　　者：李哲夫[1,2] 陈孝平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030 [2]山东省青岛大学医学院附属青岛市市立医院肝胆外科

出　　处：《腹部外科》2008年第6期364-366,共3页Journal of Abdominal Surgery

摘　　要：目的探讨黏蛋白Muc-1基因和多重耐药因子3(MDR3)在胆囊结石形成中的作用及其相互关系。方法应用免疫组织化学法和逆转录聚合酶链反应(RT-PCR)技术检测Muc-1和MDR3在36例胆囊结石(胆囊结石组)、20例胆囊炎(胆囊炎组)和12例正常胆囊(对照组)的胆囊黏膜和肝组织中的表达。结果Muc-1蛋白在胆囊结石性和胆囊炎性的胆囊黏膜中表达阳性率分别为61.1%和40.0%,非常显著高于对照组的25.0%(P<0.01);胆囊结石组显著高于胆囊炎组(P<0.05)。胆囊结石的肝组织中MDR3mRNA的表达量为(1.14±0.31),低于胆囊炎的肝组织(1.89±0.83)和正常肝组织(1.97±0.71)的表达,有非常显著性差异(P<0.01),但MDR3mRNA的表达量在后二者之间无显著性差异(P>0.05)。胆囊结石的胆囊黏膜中,Muc-1蛋白阳性表达者其肝组织中的MDR3mRNA的表达量为(0.87±0.18),与Muc-1蛋白阴性表达者(1.36±0.27)相比,有非常显著性差异(P<0.01)。结论Muc-1基因参与了胆囊结石形成的全过程。在胆囊结石形成的后期阶段,MDR3与Muc-1基因相互作用,共同影响胆囊结石的形成。Objective To evaluate the roles of mucin gene （Muc-1）and muhidrug resistance 3 （MDR3）and their interactions in cholesterol calculus formation. Methods The expression of Muc-1 and MDR3 in mucosa and liver tissue of 36 patients with cholelithiasis,20 with cholecystitis and 12 cases of normal gallbladder was detected by using immunohistochemistry and reverse transcription polymerase chain reaction（RT-PCR）. Results The positive expression rate of Muc-1 in mucosa was significantly higher in patients with cholelithiasis （61.1%）and cholecystitis （40. 0%）than that in mucosa of patients with normal gallbladder （25. 0%） （ P 〈 0. 01 ）, and also higher in patients with cholelithiasis than that in patients with cholecystitis（P〈0. 05）. The mRNA expression of MDR3 in liver were significantly lower in patients with cholelithiasis （1.14 ± 0.31）than that in liver of patients with cholecystitis （1.89 ± 0. 83）and normal gallbladder （1.97 ± 0. 71 ）respectively. However, there was no difference between cholecystitis and control group（P〉0. 05）. The MDR3 mRNA expression of liver in gallbladder mucosa positive for Muc-1 of patients with with cholelithiasis was significantly lower than that in Muc-1 negative group （0. 87 ± 0. 18 vs 1.36 ± 0. 27） （P〈0. 01 ）. Conclusion Muc-1 may be associated with the whole process of cholesterol calculus formation. In the later period, MDR3 and Muc-1 interacted and influenced the formation of cholelithiasis.

关 键 词：胆囊结石病 黏蛋白类 多药耐药相关蛋白质类 

分 类 号：R730.51[医药卫生—肿瘤] R657.44[医药卫生—临床医学]