13/17罗伯逊易位猪POU1F1基因多态性与经济性状的相关分析  

Polymorphisms of POU1F1 Gene and Their Associations with Economic Traits in 13/17 Robertsonian Translocation Pigs

作　　者：姜丽花[1] 赵雯[1] 何晓波[1] 孙金海[1] 张廷荣[1] 杜方磊[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《中国畜牧兽医》2008年第12期101-105,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金资助项目(30270948);教育部科学技术研究重点项目(J06007)

摘　　要：利用外周血淋巴细胞培养制备染色体标本,对13/17罗伯逊易位猪的3种杂交组合的394头后代猪进行分群;应用PCR-RFLP技术在POU1 F1基因的扩增片段(1746 bp)中检测到1个RsaⅠ限制性内切酶的多态酶切位点A(3种基因型);应用PCR-SSCP技术检测1个POU1 F1基因第5内含子上的突变E(2种基因型),同时结合生长和胴体品质测定记录研究基因型对生产性状的遗传效应。结果表明,位点A的基因型对生长有显著影响(P<0.05),位点E基因型对生长和眼肌面积有显著影响(P<0.05)。研究结果初步揭示POU1 F1多态性与生长、眼肌面积等存在相关,推测POU1 F1多态性可作为罗伯逊易位杂合优势遗传机制的一种分子水平上的解释。Karyotype analysis was carried out by means of culturing whole peripheral blood from 394 pigs, which were produced from three hybridization combinations of 13/17 Robertsonian translocation pig. According to karyotype all the pigs were divided into different groups. A Rsa I restriction enzyme polymorphic site （locus A） in a 1746 bp fragment of porcine POU1F1 gene was detected by using PCR-RFLP analysis and a mutation （locus E） in intron 5 of porcine POU1F1 gene was found by using PCR-SSCP analysis in three hybrid populations. At the same time, the effects on growth traits and carcass traits of POU1F1 gene were estimated according to their growth and slaughter data. The result of population genetics analyses demonstrated a significant effect on growth in locus A and a significant association with growth and loin eye area for locus E （P〈0.05）. The results indicated that growth and loin eye area were correlated with polymorphisms of POUIF1 gene which could be used as an interpretation for genetic mechanism on heterozygous advantage of Robertsonian translocation at the molecular level.

关 键 词：猪 罗伯逊易位 POU1F1基因 核型 

分 类 号：Q343.15[生物学—遗传学]