宫内感染中HBV前S/S区基因种系进化树及变异特点的研究  

The Phylogenetic Tree and Mutation Research on the HBV PreS/S Gene in HBV Intrauterine Infection

作　　者：张治国[1] 吴琳[2] 苏海霞[1] 闫永平[1] 张磊[1] 李端[1] 承海[1] 许文娟[1] 卢娟[1] 徐德忠[1] 

机构地区：[1]第四军医大学流行病学教研室,陕西西安710032 [2]第四军医大学生化教研室,陕西西安710032

出　　处：《现代生物医学进展》2008年第12期2450-2452,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金重点课题(30230320)

摘　　要：目的:通过HBV前S/S区基因变异研究探讨HBV宫内感染的机制。方法:将HBsAg阳性母亲按照随访其新生儿是否发生宫内感染,分为病例组和对照组,经PCR扩增HBV DNA,基因克隆、测序,构建种系进化树分析前S/S基因的突变。结果:共得到60株HBV序列,来源于同一组的序列较为集中在树中的某枝,而且进化距离从病例组母亲、病例组患儿到对照组母亲存在由近及远的特征。结论:各组病毒株在基因序列进化上存在差异,某些突变位点在非宫内感染组母亲中的发生率较高,突变的发生可能使其所在的编码区功能发生变化而影响宫内感染的发生。Objective： To investigate the mechanism of HBV intrauterine infection by analysis of HBV preS/S gene sequence. Methods： According to whether neonate was infected by HBV in uterus or not, their HBsAg positive mothers were divided into intrauterine infection group and non-intrauterine infection group. PreS/S gene of HBV was amplified by polymerase chain reaction （PCR）, then the PCR products were cloned and sequenced. The phylogenetic tree was produced and the sequences were compared with each group. Results： All 60 HBV strains sequences were used to produce the phylogenetic tree. The sequence from the same group were centralized on the same branch of the tree. The evolutionary distance was different between each group. The evolutionary distance of group I was the shortest in the three groups, while the evolutionary distance of group III was the longest. Conclusions： All the strains had the gene mutation. Some gene position＇s mutation rate of non-intrauterine infection group was higher than that of other groups. Some gene position＇s mutation might have association with the HBV intrauterine infection by altering the function of the coded region.

关 键 词：肝炎病毒 乙型 宫内感染 基因突变 前S/S基因 

分 类 号：R392.12[医药卫生—免疫学] R711.32[医药卫生—基础医学]