糠醛渣纤维乙醇同步糖化发酵过程研究  

Study on conversion process for furfural residue manufacture to ethanol by simultaneous saccharification and fermentation

作　　者：孙亚东[1] 孙冉[1] 蒋建新[1] 朱莉伟[1] 

机构地区：[1]北京林业大学化学工程系,北京100083

出　　处：《现代化工》2008年第12期48-52,共5页Modern Chemical Industry

基　　金：国家林业局"948"引进项目(2004-4-40);教育部新世纪优秀人才支持计划(NCET-07-0082)项目

摘　　要：以过碱化处理的糠醛渣为原料,采用正交试验法进行同步糖化发酵(SSF)转化乙醇工艺条件及过程研究。通过考察反应温度、pH、纤维素酶用量和表面活性剂浓度来优化同步糖化发酵转化工艺条件。在正交优化条件基础上,进行了5 L发酵罐试验,并同步分析表征了发酵过程中还原糖浓度、乙醇浓度、酵母细胞数、纤维素含量及其结构变化。同步糖化发酵转化糠醛渣生成乙醇的优化条件为:反应温度38℃,pH 4.2,纤维素酶用量20 FPU/(g纤维素),吐温-20质量分数0.15%,酵母接种量10%。发酵罐中同步糖化发酵糠醛渣生成乙醇的转化率达到72.33%,过程分析表明反应时间为27 h时,糠醛渣糖化发酵产乙醇的转化率达到最高,比其他纤维原料的反应转化时间大大缩短。同步糖化发酵过程中,糠醛渣纤维素含量逐步降低,纤维素表观结晶度呈下降趋势,纤维素微晶尺寸减小。Furfural residue pretreated by overliming is fermented to ethanol by batch simultaneous saccharification and fermentation （SSF） in this paper. The optimal combination of factors （reaction temperature, solution pH value, loading of both cellulase and tween-20） affecting the conversion of pretreated furfural residue to ethanol is found by orthogonal design experiments.The SSF of furfural residue is experimented in a 5 L fermentor, and the data of different phases are analyzed simultaneously. The optimal conditions are as follows： 38℃ of reaction temperature, 4.2 of pH value, cellulase 20 FPU/（ g cellulose） ,0.15% of tween-20 and 10% inoculum solution. The ethanol recovery up to 72.33% （vs theory data） can be obtained in 5 L fermentor. The simultaneous analysis result indicates that the highest ethanol conversion rate appear at 27 h, which is much less than that of other cellulose material. The cellulose content and crystallinity index of fermented furfural residue decrease as the SSF proceed, and the average dimension of cellulose crystallite decreases during enzymatic hydrolysis.

关 键 词：糠醛渣 纤维乙醇 同步糖化发酵 纤维素结构 

分 类 号：TQ92[轻工技术与工程—发酵工程]