基于SSR标记的梨资源遗传多样性分析  

Genetic Diversity of Germplasm Resources of Pyrus Based on SSR Marker

作　　者：张妤艳[1,2] 吴俊[1] 张绍铃[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]江苏省农业科学院园艺研究所,南京210014

出　　处：《农业生物技术学报》2008年第6期983-989,共7页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(No.30671437);教育部科学技术研究项目(No.105089)资助

摘　　要：利用SSR标记技术对56份梨(Pyrus)资源进行了遗传多样性分析。利用筛选出的6对SSR引物共扩增40条谱带,其中多态性位点38个,多态性位点比例为95%,每对引物产生有效等位基因6.3个。各位点期望杂合度为0.0354～0.4910,平均为0.1964;有效等位基因为1.0367～1.9648,平均1.2958;香农指数平均为0.3256,说明供试梨材料的遗传多样性较低。利用SSR标记可将44个栽培品种区分开,但无法区分芽变和原种。根据SSR标记揭示的多态性,采用NTSYS-pc2.01软件,以UP-GMA法进行聚类分析,结果显示,所检测的56份梨材料在相似系数0.71处可分为4组,其中中国的白梨(Pyrus bretschneideri Rehd.)、秋子梨(P.ussuriensis Maxin.)和砂梨(P.pyrifolia Nakai.)相互交错在一起,没有独自成组。Levels of genetic diversity among 56 germplasms of Pyrus were analyzed by SSR markers. Forty bands were amplified by six primer paris, of which 38 （95%） were polymorphic, with an average of 6.3 alleles per locus. Nei＇s gene diversity index ranged from 0.0354 to 0.4910 with an average of 0.1964, effective number of alleles ranged froml.0367 to 1.9648 with an average of 1.2958. Genetic diversity difference among tested pear cultivars was relatively low based on Shannon index （0.3256）. Forty and five pear culti- vars could be distinguished by SSR markers except the bud mutant cultivars. UPGMA cluster analysis was performed by soft NTYSIS-pc2.01, the clades formed four populations at the dice coefficient of 0.71. Pyrus bretschneideri Rehd., P. ussuriensis Maxim. and P. pyrifolia Nakai. originated in China interveined each other and could not form group independently.

关 键 词：梨 SSR 遗传多样性 亲缘关系 

分 类 号：S188[农业科学—农业基础科学]