Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species  

作　　者：So-hyung KIM Kyoung-ah KANG Rui ZHANG Mei-jing PIAO Dong-ok KO Zhi-hong WANG Sung-wook CHAE Sam-sik KANG Keun-hwa LEE Hee-kyoung KANG Hyun-wook KANG Jin-won HYUN 

机构地区：[1]School of Medicine and Institute of Medical Science, Cheju National University, Jeju-si Republic of Korea [2]Department of Herbal Resources Research, Korea Institute of Oriental Medicine, Daejeon, Republic of Korea [3]Natural Products Research Institute and College of Pharmacy, Seoul National University, Seoul, Republic of Korea

出　　处：《Acta Pharmacologica Sinica》2008年第11期1319-1326,共8页中国药理学报（英文版）

摘　　要：Aim： To investigate the anti-oxidant properties of esculetin （6,7-dihydroxycoumarin） against H2O2-induced Chinese hamster lung fibroblast （V79-4） damage. Methods： The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl （DPPH） radical, hydroxyl radical, and intracellular reactive oxygen species （ROS）. In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-（4,5-dimethylthiazole-2- yl）-2,5-diphenyltetrazolium bromide assay. Results： Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2. Conclusion： Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.Aim： To investigate the anti-oxidant properties of esculetin （6,7-dihydroxycoumarin） against H2O2-induced Chinese hamster lung fibroblast （V79-4） damage. Methods： The radical scavenging activity was assessed by 1,1-diphenyl- 2-picrylhydrazyl （DPPH） radical, hydroxyl radical, and intracellular reactive oxygen species （ROS）. In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immunofluorescence image. Cell viability was assessed by 3-（4,5-dimethylthiazole-2- yl）-2,5-diphenyltetrazolium bromide assay. Results： Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2. Conclusion： Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.

关 键 词：anti-oxidant activity ESCULETIN oxidative stress cytoprotective activity reactive oxygen species 

分 类 号：R97[医药卫生—药品]