Resveratrol protects bone marrow mesenchymal stem cell derived chondrocytes cultured on chitosan-gelatin scaffolds from the inhibitory effect of interleukin-1β  

作　　者：Ming LEI Shi-qing LIU Yu-lan LIU 

机构地区：[1]Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China [2]Department of Plastic Surgery, The Third Hospital of Wuhan, Wuhan 430060, China

出　　处：《Acta Pharmacologica Sinica》2008年第11期1350-1356,共7页中国药理学报（英文版）

摘　　要：Aim： To investigate the effects of resveratrol on interleukin-lbeta （IL-1β） induced catabolism in bone marrow mesenchymal stem cell （MSC） derived chondrocytes cultured on chitosan-gelatin scaffolds （CGS）. Methods： The chondrogenesis of alginate-encapsulated MSCs was evaluated by toluidine blue staining, RT-PCR, and immunostaing. MSC-derived chondrocyte morphology cultured on CGS was evaluated by a scanning electron microscope （SEM） and a laser confocal microscope （LCM）. When these cells on CGS were pre-stimulated with IL-1 β or co-treated with IL-1 β and resveratrol in the absence and presence of the specific β1-integrin blocking antibody, collagen type Ⅱ, aggrecan, matrix metalloproteinase-13 （MMP-13） expression, and the translocation of nuclear factor kappaB （NF-κB） were analyzed by Western blot analysis. Results： Transforming growth factor beta 3 （TGF-β3） combined with insulin-like growth factor Ⅰ （IGF-Ⅰ） induced the cartilage-specific collagen type Ⅱ, aggrecan expression and extracellular matrix （ECM） accumulation at the end of a 3-week culture. CGS supported those differentiated chondrocytes＇ attachment, proliferation, migration, and ECM formation. When those cells cultured on CGS were stimulated with IL-1β alone, collagen type Ⅱ and aggrecan expression was inhibited. However, MMP-13 expression increased. Resveratrol reversed the catabolic effects by reducing the translocation of NF-κB. A specific β1-integrin blocking antibody abrogated the effects of resveratrol on IL-1 β stimulated MSC-derived chondrocytes. Conclusion： These results indicated that resveratrol acts as a NF-κB inhibitor to protect MSC-derived chondrocytes on the CGS from the IL-1β catabolism and these effects are mediated by β1-integrin.Aim： To investigate the effects of resveratrol on interleukin-lbeta （IL-1β） induced catabolism in bone marrow mesenchymal stem cell （MSC） derived chondrocytes cultured on chitosan-gelatin scaffolds （CGS）. Methods： The chondrogenesis of alginate-encapsulated MSCs was evaluated by toluidine blue staining, RT-PCR, and immunostaing. MSC-derived chondrocyte morphology cultured on CGS was evaluated by a scanning electron microscope （SEM） and a laser confocal microscope （LCM）. When these cells on CGS were pre-stimulated with IL-1 β or co-treated with IL-1 β and resveratrol in the absence and presence of the specific β1-integrin blocking antibody, collagen type Ⅱ, aggrecan, matrix metalloproteinase-13 （MMP-13） expression, and the translocation of nuclear factor kappaB （NF-κB） were analyzed by Western blot analysis. Results： Transforming growth factor beta 3 （TGF-β3） combined with insulin-like growth factor Ⅰ （IGF-Ⅰ） induced the cartilage-specific collagen type Ⅱ, aggrecan expression and extracellular matrix （ECM） accumulation at the end of a 3-week culture. CGS supported those differentiated chondrocytes＇ attachment, proliferation, migration, and ECM formation. When those cells cultured on CGS were stimulated with IL-1β alone, collagen type Ⅱ and aggrecan expression was inhibited. However, MMP-13 expression increased. Resveratrol reversed the catabolic effects by reducing the translocation of NF-κB. A specific β1-integrin blocking antibody abrogated the effects of resveratrol on IL-1 β stimulated MSC-derived chondrocytes. Conclusion： These results indicated that resveratrol acts as a NF-κB inhibitor to protect MSC-derived chondrocytes on the CGS from the IL-1β catabolism and these effects are mediated by β1-integrin.

关 键 词：mesenchymal stem cells alginate chondrogen-esis IL-1β RESVERATROL Β1-INTEGRIN 

分 类 号：R68[医药卫生—骨科学]