Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells  

作　　者：Zhi-gang ZHUANG Fei FEI Ying CHEN Wei JIN 

机构地区：[1]Department of Breast Surgery, Shanghai First Maternity and Infant Health Hospital, Tongji University, Shanghai 200040, China [2]Department of Breast Surgery, Breast Cancer Institute, Cancer Hospital/Cancer Institute, Fudan University, Shanghai 200032, China

出　　处：《Acta Pharmacologica Sinica》2008年第12期1459-1466,共8页中国药理学报（英文版）

基　　金：This work was supported by the grant from the National Natural Science Foundation of China （No 30200151） and the Shanghai Pujiang Program and Innovation Program of Shanghai Municipal Education Commission （No 09ZZ04）.

摘　　要：Aim： To study the effects of suberoyl bis-hydroxamic acid （SBHA）, an inhibitor of histone deacetylases, on the apoptosis of MCF-7 breast cancer cells. Methods： Apoptosis in MCF-7 cells induced by SBHA was demonstrated by flow cytometric analysis, morphological observation, and DNA ladder. Mitochondrial membrane potential （△ψm） was measured using the fluorescent probe JC-1. The expressions of p53, p21, Bax, and PUMA were determined using RT-PCR or Western blotting analysis after the MCF-7 cells were treated with SBHA or p53 siRNA. Results： SBHA induced apoptosis in MCF-7 cells. The expressions of p53, p21, Bax, and PUMA were induced, and △ψm collapsed after treatment with SBHA. p53 siRNA abrogated the SBHA-induced apoptosis and the expressions of p53, p21, Bax, and PUMA. Conclusion： The activation of the p53 pathway is involved in SBHA-induced apoptosis in MCF-7 cells.Aim： To study the effects of suberoyl bis-hydroxamic acid （SBHA）, an inhibitor of histone deacetylases, on the apoptosis of MCF-7 breast cancer cells. Methods： Apoptosis in MCF-7 cells induced by SBHA was demonstrated by flow cytometric analysis, morphological observation, and DNA ladder. Mitochondrial membrane potential （△ψm） was measured using the fluorescent probe JC-1. The expressions of p53, p21, Bax, and PUMA were determined using RT-PCR or Western blotting analysis after the MCF-7 cells were treated with SBHA or p53 siRNA. Results： SBHA induced apoptosis in MCF-7 cells. The expressions of p53, p21, Bax, and PUMA were induced, and △ψm collapsed after treatment with SBHA. p53 siRNA abrogated the SBHA-induced apoptosis and the expressions of p53, p21, Bax, and PUMA. Conclusion： The activation of the p53 pathway is involved in SBHA-induced apoptosis in MCF-7 cells.

关 键 词：suberoyl bis-hydroxamic acid APOPTOSIS p53-pathway 

分 类 号：R737.9[医药卫生—肿瘤]