p38MAPK在大鼠实验性肝纤维化发生中的表达及其意义  

Expression and its location of p38MAPK in CCl_4-induced hepatic fibrosis in rats

作　　者：吴文娟[1] 杨妙芳[1] 许小兵[1] 张晓华[1] 季洪赞[1] 袁柏思[1] 朱人敏[1] 

机构地区：[1]南京大学医学院临床学院中国人民解放军南京军区南京总医院消化内科,江苏省南京市210002

出　　处：《世界华人消化杂志》2008年第34期3822-3827,共6页World Chinese Journal of Digestology

基　　金：南京军区南京总医院科研基金重点资助项目;No.2005029~~

摘　　要：目的:观察p38MAPK在实验性大鼠肝纤维化(hepatic fibrosis,HF)发生过程中表达量的变化及其定位,从而揭示p38MAPK信号传导通路与HF形成之间的关系.方法:采用CCl4sc诱导大鼠HF模型,36只♂SD大鼠(体质量在180-220g)随机分为正常对照组(12只)和CCl4造模组(24只),造模3、6、9wk结束时分别随机处死各组大鼠.取其肝脏观察HF形成过程中不同时间段各组大鼠肝组织病理变化,用RT-PCR技术检测p38MAPK mRNA在造模过程中肝组织中的表达变化,免疫组化方法检测p38MAPK在造模过程中肝组织中蛋白表达量的变化,及其在肝组织中的表达分布情况.结果:与正常对照组比较,CCl4诱导的实验性大鼠HF不同时间段纤维化程度明显加重,随着HF的形成,p38MAPK在mRNA水平和蛋白水平都表现出增加的趋势,并且主要表达于肝脏的间质细胞,肝细胞未见染色.结论:p38MAPK在HF的形成中持续上调,参与HF形成的病理过程,p38MAPK信号传导通路活化可能促进CCl4诱导的HF的形成.AIM： To investigate the expression and its location of p38MAPK in CC14-induced hepatic fibrosis in rats and hence to reveal the relationship between hepatic fibrosis and the p38 MAPK signal transduction. METHODS： A rat model of hepatic fibrosis was established by subcutaneous injection of carbonte trachloride （CCl4）. Thirty-six male SD rats （weight 180-220 g） were randomly divided into two groups： normal control group （n = 12） and model group （n = 24）. They were randomly scarified at 3, 6 and 9 weeks after injection of CCl4 respectively. Their liver tissues were analyzed for histopathological changes. Gene expression of p38MAPK was measured by reverse transcription-PCR and immunohistochemistry was used to detect the protein expression and localization. RESULTS： The level of fibrosis was markedly aggravated at different time spots in the process of CCla-induced hepatic fibrosis in rats. With the aggravation of hepatic fibrosis, the expression of p38MAPK mRNA and p38MAPK protein increased gradually, mainly in the mesenchymal cells. CONCLUSION： Up-regulated p38MAPK is involved in CCl4-induced hepatic fibrosis in rats, and p38MAPK signal transduction may promote CCl4-induced hepatic fibrosis in rats.

关 键 词：P38MAPK 肝纤维化 肝星状细胞 

分 类 号：R575.2[医药卫生—消化系统]