抗肿瘤海洋放线菌ACMA006发酵条件的优化  

Studies on the optimum fermentation conditions of marine actinomycete ACMA006 with antitumor activity

作　　者：袁献温[1] 杨瑞丽[1] 曹雪[1] 

机构地区：[1]东南大学医学院病原生物学和免疫学系,江苏南京210009

出　　处：《中国海洋药物》2008年第6期5-9,共5页Chinese Journal of Marine Drugs

基　　金：江苏省卫生厅课题(H200758)

摘　　要：目的为提高海洋放线菌ACMA006抗肿瘤活性物质的产量而对其发酵条件进行优化。方法包括对发酵培养基的组成、种龄、温度、通气量和发酵时间等条件的研究,选择最优的发酵条件。结果最佳培养基为:大豆粉10g,胰蛋白胨5g,酵母膏10g,可溶性淀粉15g,甘油15g,KH2PO40.5g,陈海水1000mL,pH7.2。最佳培养条件:温度28℃,种龄6d,装液量33%(V/V),发酵时间8d。结论在此优化条件下,菌株ACMA006的生物量为78.1g.L-1,发酵液稀释4000倍时,对人肝癌细胞HepG2的抑制率为93.7%。Objective To increase the yield of antitumor substances from marine actinomycete ACMA006 strain. Methods Fermentation conditions were studied, including combination of medium, the age of strain, ventilatory capacity and cultivation time. Results The results showed that the best medium was as following： soya powder 10g, tryptone 5g, yeast extract paste 10g, soluble starch 15g, glycerol 15g, KH2PO4 0.5g, sea water 1000mL, pH7. 2. The best culture conditions were temperature 28℃, the age of the strain 6d, the liquid volume of culture medium 50mL in 150mL Triangular flask, and cultivation time 8d. Conclusion At last 78. 1g· L^-1 biomass was obtained and the inhibition rate on human liver cancer cells HepG2 was 93.7% when the broth was diluted 4000 times.

关 键 词：海洋放线菌 抗肿瘤活性物质 发酵 优化 

分 类 号：R931.711[医药卫生—生药学] TQ460[医药卫生—药学]