废水反硝化生物反应器中喹啉降解细菌的分离与特性  

Isolation and Characteristics of Quinoline Degrading Bacteria from Wastewater Denitrifying Bioreactor

作　　者：洪璇[1] 张晓君[1] 孟智奇[1] 林振华[1] 刘彬彬[1] 朱晨光[1] 赵立平[1] 

机构地区：[1]上海交通大学生命科学技术学院、教育部微生物代谢重点实验室,上海200240

出　　处：《应用与环境生物学报》2008年第6期803-808,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.20677041);“863”计划重点项目(No.2007AA021301);上海市国际合作项目(No.05SR07107);上海市重点学科建设项目(No.B203)资助~~

摘　　要：杂环化合物喹啉是焦化等工业废水中的一种难降解化合物.有关喹啉微生物降解的研究还很有限,分离筛选多样的喹啉降解细菌,对认识喹啉的降解机制和强化废水中喹啉的降解具有重要意义.本研究通过富集和多种分离条件的培养,从焦化废水活性污泥及喹啉驯化的生物膜样品中分离获得56株与喹啉降解相关的菌株,以16SrDNA双酶切方法进行ARDRA分型,将这些菌株分为12个OTU.选取部分代表菌株进行16SrDNA测序分析,表明分离所获得的菌株主要是Ochrobactrum、Bacillus、Pseudomonas和Rhodococcus属的微生物.通过对部分菌株测定其喹啉降解能力,发现大部分菌株都能以喹啉为唯一碳源进行生长并高效降解喹啉,极少数菌株不能单独降解喹啉.降解喹啉的Ochrobactrum属菌株还未见报道.Quinoline, a nitrogenous heterocyclic compound （NHC）, is a common pollutant in many fossil industries wastewaters, such as coking plant wastewater. Due to the limitation of knowledge for the diversity of quinoline degrading bacteria, the isolation of diverse degrading bacteria from quinoline contaminated environment is important work not only for understanding the process of quinoline degradation, but also for optimizing the wastewater treatment. In this study, 56 strains of quinoline degrading bacteria were isolated from quinoline acclimated bioreactor and coking wastewater treatment bioreactor under different culture conditions. These strains were grouped into 12 OTUs by ADRDA and the representative sequences of these OTUs were affiliated to Ochrobactrum, Bacillus, Pseudomonas and Rhodococcus. Some of these strains were used for measuring their quinoline degrading capabilities. Most of the assayed strains showed high degrading efficiency, except that a few strains could not degrade quinoline independently. As we know, it is the first report that the bacteria of Ochrobactrum could degrade quinoline so far. Fig 2, Tab 3, Ref31

关 键 词：喹啉 反硝化 降解率 分离菌 16S RDNA 废水 

分 类 号：X172[环境科学与工程—环境科学] TS251.1[轻工技术与工程—农产品加工及贮藏工程]