碳源和氮源对黑曲霉分泌β-葡萄糖苷酶的影响  

Effects of carbon and nitrogen sources on β-glucosidase biosynthesis by Aspergillus niger nl-1

作　　者：赵林果[1] 游丽金[1] 倪浩[1] 宗雅冬[1] 余世袁[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2008年第6期1-4,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省自然科学基金资助项目(BK2008423);引进国际先进农业科学技术项目(2003-4-35)

摘　　要：以黑曲霉(Aspergillus nigernl-1)为产酶菌种,研究了碳源和氮源对β-葡萄糖苷酶合成的影响。结果表明:麸皮为最好的碳源,适宜的使用量为4%;(NH4)2SO4、蛋白胨分别为较佳的无机氮源和有机氮源,由无机氮与有机氮组成的复合氮源更有利于β-葡萄糖苷酶分泌,其适宜比例为0.2%(NH4)2SO4和3.19%蛋白胨。通过碳源、氮源的优化,β-葡萄糖苷酶活力达到4.82 U/mL。Effects of carbon and nitrogen sources on the biosynthesis of β - glucosidase by Aspergfllus niger nl - 1 were investigated. The results showed that bran was the best carbon source for β - glucosidase production among the studied carbon sources, and its preferable concentration was 4%. In the experimental sources of nitrogen, （NH4 ）2SO4 and the peptone were the best nitrogen source to produce β -glucosidase as inorganic nitrogen and organic nitrogen, respectively. Moreover, the inducing β - glucosidase ability of mixture nitrogen source was better than that of a single nitrogen source. The favorable ratio of them was 0. 2% ammonium sulfate and 3. 19% peptone. In these cases, the highest activity of β - glucosidase, 4.82 U/mL, was obtained.

关 键 词：Β-葡萄糖苷酶 碳源 氮源 黑曲霉 

分 类 号：TQ352[化学工程]