蔗糖蜜生产酵母中磷的吸光光度测定法  

Absorptiophotometric Determination of Phosphorus in Yeast from Sugar Cane Molasses

作　　者：许河峰[1] 吴湛霞[1] 谢建英[1] 

机构地区：[1]广东海洋大学理学院应用化学系,广东省湛江市524025

出　　处：《职业与健康》2009年第2期148-149,共2页Occupation and Health

摘　　要：目的探讨蔗糖蜜生产酵母中磷测定的样品制备与吸光光度分析方法。方法利用三氯乙酸、TritonX-100、柠檬酸混合溶液对样品的磷含量进行有效消解、浸提,并用吸光光度法进行测定。结果该方法测定结果满意,平均回收率(n=5)达96.4%～101.6%,变异系数为1.4%～1.6%。结论该样品处理及测定方法简单方便、经济实用。[Objective] To explore the sample preparation and absorptiophotometric determination of phosphorus in yeast from sugar cane molasses.[Methods] Phosphorus in samples were determined by absorptiophotometry after effective digestion and lixiviation by using a mixed solution of trichoroacetic acid, Triton X-100 and citric acid. [Results] The method obtained a satisfactory result. The average recovery rates of phosphorus were 96.4% - 101.6% （ n = 5 ） , and variation coefficient were 1.4%-1.6%.[Conclusion] The method was simple, easy to handle, economical and applicable.

关 键 词：磷 酵母 样品消解 吸光光度法 

分 类 号：R115[医药卫生—公共卫生与预防医学]