NRP-1对喉癌Hep-2细胞增殖侵袭及体外癌细胞促血管形成的影响  

The effect of NRP-1 on priliferation,invasion and tumor angiogenesis of human laryngocarcinoma cell line Hep-2

作　　者：龚宇[1] 胡国华[1] 汤为学[2] 董蒲江[2] 黄春蓓[3] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016 [2]重庆医科大学附属第一医院神经病学实验室,重庆400016 [3]重庆市第九人民医院,重庆400700

出　　处：《重庆医科大学学报》2008年第12期1479-1484,共6页Journal of Chongqing Medical University

基　　金：重庆市自然基金(2006BB5294);重庆市卫生局基金项目(072070)

摘　　要：目的:探讨NRP-1对喉癌Hep-2细胞株的增殖迁移、侵袭及体外血管形成的功能的影响,为研究NRP-1在喉癌发生发展过程中的作用提供依据。方法:用MTT法、细胞运动实验、细胞侵袭实验及体外血管生成实验分别检测NRP-1抗体组及对照组Hep-2细胞株增殖、粘附迁移、侵袭能力及体外促血管形成的能力并进行比较。结果:NRP-1抗体干预组Hep-2细胞增殖抑制率随NRP-1抗体浓度的增加而升高(r=0.624,P<0.01)。NRP-1抗体处理48h后细胞迁移能力减弱、细胞粘附能力明显减弱、侵袭力明显下降,均与对照组差异有显著性(P<0.01),在体外基质胶上诱导血管细胞形成的管腔也明显减少(P<0.01)。结论:NRP-1抗体有效抑制了喉癌Hep-2细胞株的增殖、粘附、迁移、侵袭能力及癌细胞促体外血管形成的能力,证实NRP-1不仅在肿瘤血管生成中起到重要作用,其对癌细胞自身增殖及迁移也有显著影响,提示NRP-1在喉癌发生发展过程中起到重要作用,拮抗NRP-1可能成为肿瘤治疗新途径。objective： To identify the function of NRP-1 in progression and angiogenesis of laryngocarcinoma cell line Hep-2 in vitro, methods： MTT,the cell migrate,attack and vascular formation experiments in vitro was used to evaluate the effect of NRP-1 antigen on the migrate,attack and angiogenesis capability of laryngocarcinoma cell line Hep-2. Results： The proliferation inhibition ratio of Hep-2 cells was risen up as the NRP-1 antigen＇s concentration was increaced（r=-0.624,P〈0.01）. The number of cells migrated in the group with NRP-1 antigen was fewer than that in the control group（P〈0.01 ）. After Hep-2 cells were treated with NRP-1 antigen for 48 hours,compared with the control group,the adhesion, invasion ability was significantly weakened（P〈0.01） and induction of vessel lumina formation on matrigel was much less in vitro（P〈0.01）. Conclustion： The NRP-1 antigen suppressed the Hep-2 cell line＇s proliferation, adhesion, migration,invasion, and angiogenesis in vitro, confirmed that NRP-1 not only played an impotant role in vascular endothelial cell angiogenesis,but also had remarkable influence on Hep-2 cells. It suggested that NRP-1 may plays an impotant role in the laryngocarcinoma＇s tumorigenesis and developing process, inhibit the expression of NRP-1 may be a new stratage in the treatment of laryngocarcinoma

关 键 词：喉肿瘤 神经纤毛蛋白-1 肿瘤形成 血管生成 

分 类 号：R739.65[医药卫生—肿瘤]